Cyclin E1 antibody (AA 375-411)

Catalog No. ABIN670311

Product Details anti-Cyclin E1 Antibody

Target: Cyclin E1 (CCNE1)

Binding Specificity: AA 375-411

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin E1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from rat Cyclin E

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Cyclin E1 antibody (ABIN670311)

Zhang, Yang, Li, Xu, Yan, Gao, Jia, Duan: "Angelica polysaccharides inhibit the growth and promote the apoptosis of U251 glioma cells in vitro and in vivo." in: Phytomedicine : international journal of phytotherapy and phytopharmacology, Vol. 33, pp. 21-27, (2017) (PubMed).

Chang, Niu, Gu, Zhao, Wang, Jia, Li, Xu: "Analysis of the ways and methods of signaling pathways in regulating cell cycle of NIH3T3 at transcriptional level." in: BMC cell biology, Vol. 16, pp. 25, (2016) (PubMed).

Xu, Zhang, Li, Li, Zhang: "Concentration-Dependent Diversifcation Effects of Free Cholesterol Loading on Macrophage Viability and Polarization." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 37, Issue 2, pp. 419-31, (2015) (PubMed).

Chen, Wang, Wang, Huang, Zhang: "STAT1 inhibits human hepatocellular carcinoma cell growth through induction of p53 and Fbxw7." in: Cancer cell international, Vol. 15, pp. 111, (2015) (PubMed).

Lv, Ren, Ma, Xu, Qu, Liu, Zhou, Chen, Yu: "Synthesis, biological evaluation and mechanism studies of deoxytylophorinine and its derivatives as potential anticancer agents." in: PLoS ONE, Vol. 7, Issue 1, pp. e30342, (2012) (PubMed).

Target Details for Cyclin E1

Target: Cyclin E1 (CCNE1)

Alternative Name: Cyclin E (CCNE1 Products)

Synonyms: CCNE1 antibody, ccne antibody, cyclinE antibody, CCNE antibody, AW538188 antibody, CycE1 antibody, CYCLE antibody, Ccne antibody, cyce2 antibody, cycE3 antibody, cyce antibody, fa18e07 antibody, wu:fa18e07 antibody, cyclin E1 antibody, cyclin E1 L homeolog antibody, cyclin E1 S homeolog antibody, CCNE1 antibody, ccne1 antibody, Ccne1 antibody, ccne1.L antibody, ccne1.S antibody

Background:

Synonyms: Ccne, CYCLE, G1/S-specific cyclin-E1, Ccne1

Background: The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been described. Two additional splice variants were reported but detailed nucleotide sequence information is not yet available. Transcript Variant: This variant (1) contains a different 5' end region, which includes an upstream in-frame translation start codon, when compared to variant 2. The encoded protein has a 15 aa longer N-terminus, as compared to isoform 2.

Gene ID: 25729

UniProt: P39949

Pathways: Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Mitotic G1-G1/S Phases