Cyclin B1 antibody (AA 271-433)

Catalog No. ABIN670296

Product Details anti-Cyclin B1 Antibody

Target: Cyclin B1 (CCNB1)

Binding Specificity: AA 271-433

Reactivity: Human, Mouse, Rat, Cow, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin B1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Chicken, Cow, Human, Mouse, Rat

Predicted Reactivity: Cow

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CCNB1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Cyclin B1 antibody (ABIN670296)

Wang, Wei, Xiao, Xue, Du, Liu, Xie: "Methamphetamine induces hepatotoxicity via inhibiting cell division, arresting cell cycle and activating apoptosis: In vivo and in vitro studies." in: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, Vol. 105, pp. 61-72, (2017) (PubMed).

Ghate, Das, Chaudhuri, Panja, Mandal: "Sundew plant, a potential source of anti-inflammatory agents, selectively induces G2/M arrest and apoptosis in MCF-7 cells through upregulation of p53 and Bax/Bcl-2 ratio." in: Cell death discovery, Vol. 2, pp. 15062, (2016) (PubMed).

Qu, Zou, Zhang, Sheng, Wang, Wang, Wang, Ji: "Chelidonine induces mitotic slippage and apoptotic-like death in SGC-7901 human gastric carcinoma cells." in: Molecular medicine reports, Vol. 13, Issue 2, pp. 1336-44, (2016) (PubMed).

Zhang, Pan, Xu, Niu, Ma, Xu: "Interleukin 18 augments growth ability via NF-?B and p38/ATF2 pathways by targeting cyclin B1, cyclin B2, cyclin A2, and Bcl-2 in BRL-3A rat liver cells." in: Gene, Vol. 563, Issue 1, pp. 45-51, (2015) (PubMed).

Wang, Wang: "Studying the relationship between cell cycle and Alzheimer's disease by gold nanoparticle probes." in: Analytical biochemistry, Vol. 489, pp. 32-7, (2015) (PubMed).

Guo, Cui, Peng, Fang, Zuo, Deng, Wang, Wu, Chen, Deng: "Dietary NiCl₂ causes G₂/M cell cycle arrest in the broiler's kidney." in: Oncotarget, Vol. 6, Issue 34, pp. 35964-77, (2015) (PubMed).

Dai, Ye, Peng, Zhou, Bai, Xiao, Ma, Zhou, Tang, Yao, Cao, Qin, Liu, Tan: "Aptamer TY04 inhibits the growth of multiple myeloma cells via cell cycle arrest." in: Tumour biology, Vol. 35, Issue 8, pp. 7561-8, (2014) (PubMed).

Ghate, Chaudhuri, Sarkar, Sajem, Panja, Rout, Mandal: "An antioxidant extract of tropical lichen, Parmotrema reticulatum, induces cell cycle arrest and apoptosis in breast carcinoma cell line MCF-7." in: PLoS ONE, Vol. 8, Issue 12, pp. e82293, (2013) (PubMed).

Target Details for Cyclin B1

Target: Cyclin B1 (CCNB1)

Alternative Name: Cyclin B1 (CCNB1 Products)

Synonyms: ccnb antibody, cycb antibody, cb267 antibody, cycb1 antibody, wu:fa19g04 antibody, wu:fb16d01 antibody, wu:fb16e07 antibody, wu:fi21c01 antibody, ccnb1 antibody, MGC53596 antibody, CCNB antibody, Ccnb1-rs1 antibody, Ccnb1-rs13 antibody, CycB1 antibody, Cycb-4 antibody, Cycb-5 antibody, Cycb1-rs1 antibody, cyclin B1 antibody, cyclin B1 S homeolog antibody, ccnb1 antibody, ccnb1.2 antibody, ccnb1.S antibody, CCNB1 antibody, Ccnb1 antibody, ccnb1.2.S antibody

Background:

Synonyms: CycB1, Cycb-4, Cycb-5, Ccnb1-rs1, Cycb1-rs1, Ccnb1-rs13, G2/mitotic-specific cyclin-B1, Ccnb1, Ccn-2, Cycb

Background: The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008].

Gene ID: 268697

UniProt: P14635

Pathways: Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase