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MFN1 antibody (AA 651-741)

Name: MFN1 antibody (AA 651-741)
SKU: ABIN670071
Price: 384.62 USD
Availability: InStock

MFN1 Reactivity: Mouse, Rat, Rabbit, Chicken WB, ELISA, FACS, IHC (p) Host: Rabbit Polyclonal unconjugated

Rat brain lysates probed with Anti-Mfn1 Polyclonal Antibody at 1:5000 90min in 37˚C. (MFN1 antibody (AA 651-741))

RSC96 probed with Mfn1 Polyclonal Antibody, Unconjugated at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange). (MFN1 antibody (AA 651-741))

FACS

Paraformaldehyde-fixed, paraffin embedded mouse testis, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Mitofusin 1 Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining. (MFN1 antibody (AA 651-741))

IHC (p)

Paraformaldehyde-fixed, paraffin embedded rat testis, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Mitofusin 1 Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining. (MFN1 antibody (AA 651-741))

IHC (p)

4 images

(3 references)

Catalog No. ABIN670071

Datasheet as PDF

Target See all MFN1 Antibodies

MFN1 (Mitofusin 1 (MFN1))
Binding Specificity
15

6

4

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1
AA 651-741
Reactivity
47

30

27

20

15

5

4

4

3

2

2

1

1

1

1
Mouse, Rat, Rabbit, Chicken
Host
58

5

3

1
Rabbit
Compatible Secondaries
Clonality
53

14
Polyclonal
Conjugate
35

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
This MFN1 antibody is un-conjugated
Application
49

21

15

12

6

5

4

2

1

1

1
Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity

Chicken, Mouse, Rabbit, Rat

Predicted Reactivity

Human,Horse

Purification

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from human Mfn 1

Isotype

IgG

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MFN1 Reactivity: Human, Rabbit, Dog, Horse, Monkey IHC, IHC (p) Host: Goat Polyclonal unconjugated

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MFN1 Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Mitofusin 1 (MFN1) (AA 1-100) antibody
MFN1 Reactivity: Human WB, ELISA Host: Mouse Polyclonal unconjugated

Application Notes

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Ku, Ji, Zhang, Li, Sang: "PM2.5, SO2 and NO2 co-exposure impairs neurobehavior and induces mitochondrial injuries in the mouse brain." in: Chemosphere, Vol. 163, pp. 27-34, (2016) (PubMed).

Qin, Deng, Lou, Yu, Pei, Guan et al.: "The decreased expression of mitofusin-1 and increased fission-1 together with alterations in mitochondrial morphology in the kidney of rats with chronic fluorosis may involve elevated oxidative ..." in: Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS), (2014) (PubMed).

Lou, Guan, Liu, Liu, Zhang, Pan, Pei: "The influence of chronic fluorosis on mitochondrial dynamics morphology and distribution in cortical neurons of the rat brain." in: Archives of toxicology, Vol. 87, Issue 3, pp. 449-57, (2013) (PubMed).
Target

MFN1 (Mitofusin 1 (MFN1))

Alternative Name

Mfn1 (MFN1 Products)

Background

Synonyms: hfzo1, hfzo2, Mitofusin-1, Fzo homolog, Transmembrane GTPase MFN1, MFN1
Background: Essential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN1 acts independently of the cytoskeleton. Overexpression induces the formation of mitochondrial networks.

Gene ID

55669

UniProt

Q8IWA4