RAB7A antibody

Catalog No. ABIN6699197

Supplier Product No.: 600-401-i05

Product Details anti-RAB7A Antibody

Target: RAB7A (RAB7A, Member RAS Oncogene Family (RAB7A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAB7A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Supplier Product No.: 600-401-i05

Supplier: Rockland

Purpose: RAB7 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with Rab-7 from rat and New World monkey based on 100 % homology with the immunizing sequence.

Purification: Anti-Rab-7 Antibody was purified by peptide affinity chromatography.

Immunogen: Rab-7 Antibody was produced from whole rabbit serum prepared by repeated immunizations at the internal amino acids of human Rab-7 protein.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry_Dilution: 10 μg/mL (paraffin)

Western_Blot_Dilution: 0.5-1 μg/mL

Comment:

Anti-Rab7 antibody has been tested in IF, IHC (p), and WB. Expect a band approximately 23kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.05 % BSA

Preservative: 0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for RAB7A

Target: RAB7A (RAB7A, Member RAS Oncogene Family (RAB7A))

Alternative Name: Rab-7 (RAB7A Products)

Background:

Synonyms: RAB7, Ras-related protein Rab-7a

Background: Anti-Rab7 antibody detects human Rab7. Rab7 is a 207 amino acid transport carrier, belonging to the Rab family of RAS-related GTP-binding proteins with 4 GTP/GDP-binding sites and terminates in sequences such as cys-X-cys, cys-cys or a closely related sequence. Rab7 located in specific intracellular compartments is known to be an important regulator of vesicular transport of proteins from early to late endosomes and lysosome biogenesis. Rab7 accelerates thyroid hormone production controlling aggregation and fusion of late lysosomes. It is ubiquitously expressed in most of the tissues and plays an essential role in maintenance of the perinuclear lysosome compartment. Anti-Rab7 antibody is ideal for investigators involved in caner, NFkappaB , and signaling research, kinase and phosphatase protein research.

Gene Name: RAB7A

Gene ID: 7879

NCBI Accession: NP_004628

UniProt: P51149

Pathways: EGFR Signaling Pathway, Maintenance of Protein Location, SARS-CoV-2 Protein Interactome