Chromogranin A antibody (AA 420-457)

Catalog No. ABIN669846

Product Details anti-Chromogranin A Antibody

Target: Chromogranin A (CHGA)

Binding Specificity: AA 420-457

Reactivity: Human, Rat, Mouse, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Chromogranin A antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity: Chicken, Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CGA

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Chromogranin A antibody (ABIN669846)

Liu, Zhang, Zhang, Zhang, Hao, Song, Li, Feng, Hao, Shen, Min, Yang, Zhao: "Regulation of neuroendocrine cells and neuron factors in the ovary by zinc oxide nanoparticles." in: Toxicology letters, Vol. 256, pp. 19-32, (2016) (PubMed).

Liu, Nelson, Cromeens, Rager, Lannutti, Johnson, Besner: "HB-EGF embedded in PGA/PLLA scaffolds via subcritical CO2 augments the production of tissue engineered intestine." in: Biomaterials, Vol. 103, pp. 150-9, (2016) (PubMed).

Liu, Rager, Johnson, Enmark, Besner: "Enriched Intestinal Stem Cell Seeding Improves the Architecture of Tissue Engineered Intestine." in: Tissue engineering. Part C, Methods, (2015) (PubMed).

Wang, Ahmad, Sims, Magness, Allbritton: "In vitro generation of colonic epithelium from primary cells guided by microstructures." in: Lab on a chip, Vol. 14, Issue 9, pp. 1622-31, (2014) (PubMed).

Wang, Ahmad, Shah, Sims, Magness, Allbritton: "Capture and 3D culture of colonic crypts and colonoids in a microarray platform." in: Lab on a chip, Vol. 13, Issue 23, pp. 4625-34, (2013) (PubMed).

Target Details for Chromogranin A

Target: Chromogranin A (CHGA)

Alternative Name: Chromogranin A (CHGA Products)

Background:

Synonyms: CGA, Chromogranin-A, Pituitary secretory protein I, SP-I, CHGA

Background: The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides, vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Other peptides, including chromostatin, beta-granin, WE-14 and GE-25, are also derived from the full-length protein. However, biological activities for these molecules have not been shown. [provided by RefSeq, Jul 2008].

Gene ID: 1113

UniProt: P10645

Pathways: Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling