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MME antibody (AA 501-600)

Name: MME antibody (AA 501-600)
SKU: ABIN669666
Price: 384.62 USD
Availability: InStock

MME Reactivity: Human, Mouse, Rat FACS, WB, ELISA, ICC, IHC (p), IHC (fro), IF (p), IF (cc) Host: Rabbit Polyclonal unconjugated

Formalin-fixed and paraffin embedded mouse small intestine tissue labeled with Anti-CD10 Polyclonal Antibody (ABIN669666), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining (MME antibody (AA 501-600))

IHC

Human Hela cells probed with CD10 Polyclonal Antibody, Alexa Fluor 647 conjugated -A647) (green) at 1:20 for 30 minutes compared to unstained cells (blue) and isotype control (orange). (MME antibody (AA 501-600))

FACS

Paraformaldehyde-fixed, paraffin embedded human colon carcinoma, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with CD10 Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining (MME antibody (AA 501-600))

IHC (p)

A549 cells probed with CD10 Polyclonal Antibody, Unconjugated at 1:20 for 30 minutes followed by incubation with a conjugated secondary -PE) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange). (MME antibody (AA 501-600))

FACS

5 images

(1 reference)

Catalog No. ABIN669666

Datasheet as PDF

Target See all MME Antibodies

MME (Membrane Metallo-Endopeptidase (MME))
Binding Specificity
14

9

8

8

7

7

7

7

6

5

4

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1
AA 501-600
Reactivity
190

43

36

3

2

2

2

2

2

1

1

1

1
Human, Mouse, Rat
Host
100

95

2
Rabbit
Compatible Secondaries
Clonality
106

90

2
Polyclonal
Conjugate
105

20

16

12

9

5

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This MME antibody is un-conjugated
Application
109

79

75

65

34

27

18

17

10

8

5

5

3

2

2

1

1

1
Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Cross-Reactivity

Human, Mouse, Rat

Purification

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from human CD10

Isotype

IgG

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anti-Membrane Metallo-Endopeptidase (MME) (AA 382-538) antibody
MME Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Membrane Metallo-Endopeptidase (MME) (AA 297-483) antibody
MME Reactivity: Human WB, IHC, StM Host: Mouse Monoclonal MME-1870 unconjugated

anti-Membrane Metallo-Endopeptidase (MME) (AA 286-428) antibody
MME Reactivity: Rat WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Membrane Metallo-Endopeptidase (MME) antibody (FITC)
MME Reactivity: Human FACS Host: Mouse Monoclonal MEM-78 FITC

anti-Membrane Metallo-Endopeptidase (MME) (AA 549-750) antibody
MME Reactivity: Human FACS, WB, ELISA Host: Mouse Monoclonal 6G12E12 unconjugated

anti-Membrane Metallo-Endopeptidase (MME) (AA 297-483) antibody
MME Reactivity: Human WB, IHC, ELISA, StM, Coat Host: Mouse Monoclonal MME-1892 unconjugated

anti-Membrane Metallo-Endopeptidase (MME) (AA 52-750) antibody
MME Reactivity: Human, Mouse, Rat WB, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Membrane Metallo-Endopeptidase (MME) antibody
MME Reactivity: Human FACS, IP Host: Mouse Monoclonal MEM-78 unconjugated

Application Notes

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Chopra, Kraus, Schwinn, Ritz, Mattenheimer, Mottok, Rosenwald, Einsele, Beilhack: "Non-invasive bioluminescence imaging to monitor the immunological control of a plasmablastic lymphoma-like B cell neoplasia after hematopoietic cell transplantation." in: PLoS ONE, Vol. 8, Issue 12, pp. e81320, (2013) (PubMed).
Target

MME (Membrane Metallo-Endopeptidase (MME))

Alternative Name

CD10 (MME Products)

Synonyms

CALLA antibody, CD10 antibody, NEP antibody, SFE antibody, 6030454K05Rik antibody, C85356 antibody, Nep antibody, neprilysin antibody, membrane metalloendopeptidase antibody, membrane metallo endopeptidase antibody, membrane metallo-endopeptidase antibody, MME antibody, Mme antibody

Target Type

Chemical

Background

Synonyms: NEP, SFE, CD1, CALLA, Neprilysin, Atriopeptidase, Common acute lymphocytic leukemia antigen, Enkephalinase, Neutral endopeptidase 24.11, Neutral endopeptidase, Skin fibroblast elastase, MME, EPN
Background: Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 3 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers.

Gene ID

4311

UniProt

P08473

Pathways

RTK Signaling, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Smooth Muscle Cell Migration