SOX2 antibody (AA 227-317)

Catalog No. ABIN669621

Product Details anti-SOX2 Antibody

Target: SOX2 (SRY (Sex Determining Region Y)-Box 2 (SOX2))

Binding Specificity: AA 227-317

Reactivity: Human, Mouse, Rat, Goat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SOX2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Goat, Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Sheep,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from mouse Transcription factor SOX-2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-SOX2 antibody (ABIN669621)

Wang, Wang, Li, Liu, Jiang, Li, Cao, Zhao, Wang, Luo: "Silencing stem cell factor attenuates stemness and inhibits migration of cancer stem cells derived from Lewis lung carcinoma cells." in: Tumour biology, Vol. 37, Issue 6, pp. 7213-27, (2016) (PubMed).

Yan, Li, Li, Lin, Yang: "Osthole Protects Bone Marrow-Derived Neural Stem Cells from Oxidative Damage through PI3K/Akt-1 Pathway." in: Neurochemical research, (2016) (PubMed).

Ma, Zhou, Li, Zheng, Wu, Niu, Tang, He, Li, Hua: "Lin28a promotes self-renewal and proliferation of dairy goat spermatogonial stem cells (SSCs) through regulation of mTOR and PI3K/AKT." in: Scientific reports, Vol. 6, pp. 38805, (2016) (PubMed).

Liu, Zhang, Wang, Yang, Tan: "JNK is required for maintaining the tumor-initiating cell-like properties of acquired chemoresistant human cancer cells." in: Acta pharmacologica Sinica, Vol. 36, Issue 9, pp. 1099-106, (2016) (PubMed).

Liu, Jiang, Wang, Hao, Shang, Zhang, Cao, Xu, Zhan, Wang: "A panel of protein markers for the early detection of lung cancer with bronchial brushing specimens." in: Cancer cytopathology, Vol. 122, Issue 11, pp. 833-41, (2014) (PubMed).

Vadasz, Jensen, Moncada, Girard, Zhang, Blanchette, Finck: "Second and third trimester amniotic fluid mesenchymal stem cells can repopulate a de-cellularized lung scaffold and express lung markers." in: Journal of pediatric surgery, Vol. 49, Issue 11, pp. 1554-63, (2014) (PubMed).

Liu, Li, Liu, Sui, Liu, Xiao, Zheng, Chen, Yao, Xing, Zhou, Li: "A signature for induced pluripotent stem cell-associated genes in colorectal cancer." in: Medical oncology (Northwood, London, England), Vol. 30, Issue 1, pp. 426, (2013) (PubMed).

Gao, Zhao, Song, Yang: "Expression pattern of embryonic stem cell markers in DFAT cells and ADSCs." in: Molecular biology reports, Vol. 39, Issue 5, pp. 5791-804, (2012) (PubMed).

Target Details for SOX2

Target: SOX2 (SRY (Sex Determining Region Y)-Box 2 (SOX2))

Alternative Name: SOX2 (SOX2 Products)

Synonyms: ANOP3 antibody, MCOPS3 antibody, RGD1565646 antibody, Sox-2 antibody, lcc antibody, ysb antibody, cb236 antibody, wu:fb83g04 antibody, wu:fc14d07 antibody, zgc:65860 antibody, zgc:77389 antibody, SRY-box2 antibody, Xsox-2 antibody, anop3 antibody, mcops3 antibody, sox-2 antibody, SRY-box 2 antibody, SRY box 2 antibody, SRY (sex determining region Y)-box 2 antibody, SRY-box 2 S homeolog antibody, SOX2 antibody, Sox2 antibody, sox2 antibody, sox2.S antibody

Background:

Synonyms: lcc, ysb, Sox-2, Transcription factor SOX-2, Sox2

Background: Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP26. Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity).

Gene ID: 20674

UniProt: P48432

Pathways: Dopaminergic Neurogenesis, Sensory Perception of Sound, Stem Cell Maintenance, Cell RedoxHomeostasis