BrdU antibody

Catalog No. ABIN669246

Product Details anti-BrdU Antibody

Target: BrdU (Bromodeoxyuridine (BrdU))

Reactivity: Please inquire

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BrdU antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA

Cross-Reactivity: Human, Rat

Cross-Reactivity (Details): BrdU

Purification: Purified by Protein A.

Immunogen: BrdU conjugated to KLH

Isotype: IgG

Application Details

Application Notes: IHC-P 1:100-500
IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-BrdU antibody (ABIN669246)

Giuliani, Neri, Canalini, Calevro, Ottani, Vandini, Sena, Zaffe, Guarini: "NDP-?-MSH induces intense neurogenesis and cognitive recovery in Alzheimer transgenic mice through activation of melanocortin MC4 receptors." in: Molecular and cellular neurosciences, Vol. 67, pp. 13-21, (2015) (PubMed).

Chen, Li, Xu, Yin: "Sodium iodate influences the apoptosis, proliferation and differentiation potential of radial glial cells in vitro." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 34, Issue 4, pp. 1109-24, (2015) (PubMed).

Zhang, Zhang, Fang, Li, Zhao, Shi, An: "Neuroprotective effects of sulforaphane on cholinergic neurons in mice with Alzheimer's disease-like lesions." in: International journal of molecular sciences, Vol. 15, Issue 8, pp. 14396-410, (2014) (PubMed).

Target Details for BrdU

Target: BrdU (Bromodeoxyuridine (BrdU))

Alternative Name: BrdU (BrdU Products)

Target Type: Chemical

Background:

Synonyms: Bromodeoxyuridine, Bromodeoxyuridine, 5-Bromo-2-deoxyuridine, 5-BrdU, proliferation Marker.

Background: The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal is an accurate and comprehensive method to quantitate the degree of DNA-synthesis.BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis