CD31 antibody (AA 601-680)

Catalog No. ABIN669006

Product Details anti-CD31 Antibody

Key Features:

• High quality CD31 Primary Antibody for the detection of PECAM1.
• Reliable product with high standard validation data

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Binding Specificity: AA 601-680

Reactivity: Human, Mouse, Rat, Rabbit, Pig, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD31 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Cross-Reactivity: Dog, Human, Mouse, Pig, Rabbit, Rat

Predicted Reactivity: Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from mouse CD31

Isotype: IgG

Product Specific Information:

What can the CD31 Antibody ABIN669006 be used for?: This polyclonal CD31 Antibody detects CD31. The CD31 Antibody has been validated for various applications and can be used for the detection of CD31 and derivatives by Flow Cytometry, Western Blotting, ELISA, Immunohistochemistry (Paraffin-embedded Sections), Immunohistochemistry (Frozen Sections), Immunofluorescence (Paraffin-embedded Sections), Immunofluorescence (Cultured Cells).

What validation data is available for this CD31 Antibody?: The primary antibody is referenced in 15 publications and is characterized by a proven, very high reliability. It has currently 8 product images that show its performance in a variety of applications. The product is currently available in 100 μL quantity. CD31 Antibody for the detection of CD31 and derivatives.

What is the function of CD31?: The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010].

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-CD31 antibody (ABIN669006)

Li, Zhang, Chen, Tian, Wang: "Increased Angiogenic and Adipogenic Differentiation Potentials in Adipose-Derived Stromal Cells from Thigh Subcutaneous Adipose Depots Compared with Cells from the Abdomen." in: Aesthetic surgery journal, Vol. 39, Issue 5, pp. NP140-NP149, (2019) (PubMed).

Vuletic, Zhou, Li, Bai, Meng, Zhu, Ding, Li, Sun, Ren: "Validation of Bevacizumab Therapy Effect on Colon Cancer Subtypes by Using Whole Body Imaging in Mice." in: Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging, (2017) (PubMed).

Long, Lankford, Kumar, Grahn, Borjesson, Farmer, Wang: "Isolation and characterization of canine placenta-derived mesenchymal stromal cells for the treatment of neurological disorders in dogs." in: Cytometry. Part A : the journal of the International Society for Analytical Cytology, Vol. 93, Issue 1, pp. 82-92, (2017) (PubMed).

Korkmaz, Ulrich, Vogels, de Wit, van Zuijlen, Krijnen, Niessen: "Neutrophil extracellular traps coincide with a pro-coagulant status of microcirculatory endothelium in burn wounds." in: Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society, Vol. 25, Issue 4, pp. 609-617, (2017) (PubMed).

Zhang, Chen, Liu, Zhou, Hu, Zhou, Han, Chen: "Exendin-4 promotes proliferation of adipose-derived stem cells through ERK and JNK signaling pathways." in: In vitro cellular & developmental biology. Animal, Vol. 52, Issue 5, pp. 598-606, (2016) (PubMed).

Guo, Wang, Qiao, Yang, Su, Gao, Xu: "A Single Injection of Recombinant Adeno-Associated Virus into the Lumbar Cistern Delivers Transgene Expression Throughout the Whole Spinal Cord." in: Molecular neurobiology, (2015) (PubMed).

Zhou, Yang, Xin, Zhang, Hu, Zhou, Chen, Chen et al.: "Exendin-4 enhances the migration of adipose-derived stem cells to neonatal rat ventricular cardiomyocyte-derived conditioned medium via the phosphoinositide 3-kinase/Akt-stromal cell-derived ..." in: Molecular medicine reports, (2015) (PubMed).

Wang, Wang, Fang, Zhou, Liu, Su: "High glucose induces and activates Toll-like receptor 4 in endothelial cells of diabetic retinopathy." in: Diabetology & metabolic syndrome, Vol. 7, pp. 89, (2015) (PubMed).

Yu, Wang, Wang, Lu, Li, Qin, Huang: "Activation of liver X receptor enhances the proliferation and migration of endothelial progenitor cells and promotes vascular repair through PI3K/Akt/eNOS signaling pathway activation." in: Vascular pharmacology, Vol. 62, Issue 3, pp. 150-61, (2014) (PubMed).

Rossmeisl, Garcia, Roberston, Ellis, Davalos: "Pathology of non-thermal irreversible electroporation (N-TIRE)-induced ablation of the canine brain." in: Journal of veterinary science, Vol. 14, Issue 4, pp. 433-40, (2014) (PubMed).

Zeng, Zhang, Li, Yang, Chen, Liu: "A new method to isolate and culture rat kupffer cells." in: PLoS ONE, Vol. 8, Issue 8, pp. e70832, (2013) (PubMed).

Yuan, Huang, Chu, Li, Li, Si: "A targeted high-efficiency angiogenesis strategy as therapy for myocardial infarction." in: Life sciences, Vol. 90, Issue 17-18, pp. 695-702, (2012) (PubMed).

Yuan, Huang, Chu, Li, Si: "A visible, targeted high-efficiency gene delivery and transfection strategy." in: BMC biotechnology, Vol. 11, pp. 56, (2011) (PubMed).

Zhang, Huang, Wu, Yang, Song, Chen, Fan, Wang: "Cardiac stem cells differentiate into sinus node-like cells." in: The Tohoku journal of experimental medicine, Vol. 222, Issue 2, pp. 113-20, (2010) (PubMed).

Chen, Lin, Kanekura, Su, Lin, Xie, Wu, Li, Chen, Chang: "A small interfering CD147-targeting RNA inhibited the proliferation, invasiveness, and metastatic activity of malignant melanoma." in: Cancer research, Vol. 66, Issue 23, pp. 11323-30, (2006) (PubMed).

Target Details for CD31

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Alternative Name: CD31 (PECAM1 Products)

Synonyms: CD31 antibody, CD31/EndoCAM antibody, GPIIA' antibody, PECA1 antibody, PECAM-1 antibody, endoCAM antibody, C85791 antibody, Cd31 antibody, Pecam antibody, platelet/endothelial cell adhesion molecule 1 antibody, platelet and endothelial cell adhesion molecule 1 antibody, pecam1 antibody, PECAM1 antibody, Pecam1 antibody

Background:

Synonyms: CD31, PECA1, GPIIA', PECAM-1, endoCAM, CD31/EndoCAM, Platelet endothelial cell adhesion molecule, PECAM1

Background: Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-69 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).

Gene ID: 5175

UniProt: P16284

Pathways: Regulation of Actin Filament Polymerization