Ghrelin antibody (AA 31-117)

Catalog No. ABIN668991

Product Details anti-Ghrelin Antibody

Target: Ghrelin (GHRL)

Binding Specificity: AA 31-117

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ghrelin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Rat

Predicted Reactivity: Mouse,Dog,Cow,Sheep,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Ghrelin

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Ghrelin antibody (ABIN668991)

Liu, Liu, Li, Chen, Gu et al.: "Ghrelin protects the myocardium with hypoxia/reoxygenation treatment through upregulating the expression of growth hormone, growth hormone secretagogue receptor and insulin-like growth factor-1, and ..." in: International journal of molecular medicine, Vol. 42, Issue 6, pp. 3037-3046, (2019) (PubMed).

Wölnerhanssen, Moran, Burdyga, Meyer-Gerspach, Peterli, Manz, Thumshirn, Daly, Beglinger, Shirazi-Beechey: "Deregulation of transcription factors controlling intestinal epithelial cell differentiation; a predisposing factor for reduced enteroendocrine cell number in morbidly obese individuals." in: Scientific reports, Vol. 7, Issue 1, pp. 8174, (2017) (PubMed).

Dai, Jiang, Yuan, Yang, Yang, Huang: "Effects of zearalenone-diet on expression of ghrelin and PCNA genes in ovaries of post-weaning piglets." in: Animal reproduction science, Vol. 168, pp. 126-37, (2016) (PubMed).

Lemarié, Beauchamp, Dayot, Duby, Legrand, Rioux: "Dietary Caprylic Acid (C8:0) Does Not Increase Plasma Acylated Ghrelin but Decreases Plasma Unacylated Ghrelin in the Rat." in: PLoS ONE, Vol. 10, Issue 7, pp. e0133600, (2015) (PubMed).

Wang, Song, Li, Liu, Ma, Mao, Wu, Wu, Li, Guan, Liu: "Effects of Wen Dan Tang on insomnia-related anxiety and levels of the brain-gut peptide Ghrelin." in: Neural regeneration research, Vol. 9, Issue 2, pp. 205-12, (2014) (PubMed).

Target Details for Ghrelin

Target: Ghrelin (GHRL)

Alternative Name: Ghrelin (GHRL Products)

Synonyms: 2210006E23Rik antibody, Ghr antibody, MTLRP antibody, MTLRPAP antibody, m46 antibody, Gh1-1 antibody, GRHL antibody, MTLRP-AP antibody, GHR antibody, ghrelin antibody, ghrelin and obestatin prepropeptide antibody, ghrelin/obestatin prepropeptide antibody, Ghrl antibody, GHRL antibody, ghrl antibody

Background:

Synonyms: MTLRP, Appetite-regulating hormone, Growth hormone secretagogue, Growth hormone-releasing peptide, Motilin-related peptide, Protein M46, GHRL, UNQ524/PRO1066

Background: Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR). Induces the release of growth hormone from the pituitary. Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation. Obestatin may be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility (By similarity).

Gene ID: 51738

UniProt: Q9UBU3

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Peptide Hormone Metabolism, Negative Regulation of Hormone Secretion, Synaptic Membrane, Feeding Behaviour