MMP2 antibody (AA 31-109)

Catalog No. ABIN668286

Product Details anti-MMP2 Antibody

Target: MMP2 (Matrix Metalloproteinase 2 (MMP2))

Binding Specificity: AA 31-109

Reactivity: Human, Mouse, Rat, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MMP2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Mouse, Rabbit, Rat

Predicted Reactivity: Cow,Sheep,Pig,Chicken

Purification: Purified by Protein A.

Immunogen: MAT conjugated synthetic peptide derived from human MMP2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-MMP2 antibody (ABIN668286)

Zhang, Li, Song, Xiong, Liu, Bai: "Hydroxysafflor yellow A suppresses angiogenesis of hepatocellular carcinoma through inhibition of p38 MAPK phosphorylation." in: Biomedicine & pharmacotherapy, Vol. 109, pp. 806-814, (2019) (PubMed).

Gao, Li, Liu, Wang, Zhang, Li, Bu: "Effect of Telmisartan on Preventing Learning and Memory Deficits Via Peroxisome Proliferator-Activated Receptor-γ in Vascular Dementia Spontaneously Hypertensive Rats." in: Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association, Vol. 27, Issue 2, pp. 277-285, (2018) (PubMed).

Wang, Chen, Ke, Li: "Ghrelin inhibits atherosclerotic plaque angiogenesis and promotes plaque stability in a rabbit atherosclerotic model." in: Peptides, Vol. 90, pp. 17-26, (2017) (PubMed).

Varghese, Joseph, S R, B S, Sreelekha: "The inhibitory effect of anti- tumor polysaccharide from Punica granatum on metastasis." in: International journal of biological macromolecules, Vol. 103, pp. 1000-1010, (2017) (PubMed).

Vendrov, Stevenson, Alahari, Pan, Wickline, Madamanchi, Runge: "Attenuated Superoxide Dismutase 2 Activity Induces Atherosclerotic Plaque Instability During Aging in Hyperlipidemic Mice." in: Journal of the American Heart Association, Vol. 6, Issue 11, (2017) (PubMed).

Ni, Ding, Zhou, Qiu, Tang: "Renoprotective effects of berberine through regulation of the MMPs/TIMPs system in streptozocin-induced diabetic nephropathy in rats." in: European journal of pharmacology, Vol. 764, pp. 448-456, (2015) (PubMed).

Madka, Mohammed, Li, Zhang, Kumar, Lightfoot, Wu, Steele, Kopelovich, Rao: "TP53 modulating agent, CP-31398 enhances antitumor effects of ODC inhibitor in mouse model of urinary bladder transitional cell carcinoma." in: American journal of cancer research, Vol. 5, Issue 10, pp. 3030-41, (2015) (PubMed).

Ren, Fan, Mei, Wu, Liu, Pu, You, Liu: "Interferon-γ and celecoxib inhibit lung-tumor growth through modulating M2/M1 macrophage ratio in the tumor microenvironment." in: Drug design, development and therapy, Vol. 8, pp. 1527-38, (2015) (PubMed).

Zhu, Chen, Chen, Chen, Song, Sun, Zhao: "Activating transcription factor 4 promotes esophageal squamous cell carcinoma invasion and metastasis in mice and is associated with poor prognosis in human patients." in: PLoS ONE, Vol. 9, Issue 7, pp. e103882, (2014) (PubMed).

Zhang, Qi, Cao, Zu, Chen, Li, Qi: "5-Aza-2'-Deoxycytidine Enhances Maspin Expression and Inhibits Proliferation, Migration, and Invasion of the Bladder Cancer T24 Cell Line." in: Cancer biotherapy & radiopharmaceuticals, (2013) (PubMed).

Qin, Fan, Zhao, Cui, Wei, Kohama, Gordon, Li, Gao: "Recombinant human CXCL8(3-72)K11R/G31P regulates smooth muscle cell proliferation and migration through blockage of interleukin-8 receptor." in: IUBMB life, Vol. 65, Issue 1, pp. 67-75, (2013) (PubMed).

Fang, Wu, Huang: "Raloxifene upregulated mesangial cell MMP-2 activity via ER-? through transcriptional regulation." in: Cell biochemistry and biophysics, Vol. 67, Issue 2, pp. 607-13, (2013) (PubMed).

Wu, Ning, Zeng, Fan, Zhou, Zhang, Zhang, Chen, Gao, Wang, Guo, Li, Wang, He: "Silibinin inhibits ?-catenin/ZEB1 signaling and suppresses bladder cancer metastasis via dual-blocking epithelial-mesenchymal transition and stemness." in: Cellular signalling, Vol. 25, Issue 12, pp. 2625-33, (2013) (PubMed).

Wu, Fan, Zhang, Ning, Zeng, Zhou, Li, Chen, Zhang, Wang, Hsieh, He: "PI3K/Akt to GSK3?/?-catenin signaling cascade coordinates cell colonization for bladder cancer bone metastasis through regulating ZEB1 transcription." in: Cellular signalling, Vol. 24, Issue 12, pp. 2273-82, (2012) (PubMed).

Wu, Zeng, Zhou, Fan, Chen, Wang, Zhang, Wang, He: "Slug contributes to cadherin switch and malignant progression in muscle-invasive bladder cancer development." in: Urologic oncology, (2012) (PubMed).

Wu, Zeng, Li, Fan, Zhang, Xue, Zhu, Yang, Wang, He: "Silibinin reverses epithelial-to-mesenchymal transition in metastatic prostate cancer cells by targeting transcription factors." in: Oncology reports, Vol. 23, Issue 6, pp. 1545-52, (2010) (PubMed).

Target Details for MMP2

Target: MMP2 (Matrix Metalloproteinase 2 (MMP2))

Alternative Name: MMP2 (MMP2 Products)

Synonyms: 2-MMP antibody, CG1794 antibody, DM2-MMP antibody, Dm2-MMP antibody, Dmel\\CG1794 antibody, MMP2 antibody, anon-WO0118547.84 antibody, dm-2MMP antibody, dmmp2 antibody, l(2)02353 antibody, mmp2 antibody, wu:fa99h12 antibody, wu:fk89d01 antibody, fgmmp-2 antibody, Mmp-2 antibody, LOC100135793 antibody, Clg4a antibody, GelA antibody, MMP-2 antibody, CLG4 antibody, CLG4A antibody, MMP-II antibody, MONA antibody, TBE-1 antibody, matrix metallopeptidase 2 antibody, Matrix metalloproteinase 2 antibody, matrix metalloproteinase 2 antibody, matrix metalloproteinase-2 antibody, MMP2 antibody, Mmp2 antibody, mmp2 antibody, LOC657982 antibody, LOC100135793 antibody

Background:

Synonyms: CLG4, MONA, CLG4A, MMP-2, TBE-1, MMP-II, 72 kDa type IV collagenase, 72 kDa gelatinase, Gelatinase A, Matrix metalloproteinase-2, MMP2

Background: Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues in association with MMP14. PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels. Isoform 2: Mediates the proteolysis of CHUK/IKKA and initiates a primary innate immune response by inducing mitochondrial-nuclear stress signaling with activation of the pro-inflammatory NF-kappaB, NFAT and IRF transcriptional pathways.

Gene ID: 4313

UniProt: P08253

Pathways: Activation of Innate immune Response