CRISPR-Cas9 (C-Term) antibody (DyLight 549)

Catalog No. ABIN6658288

Product Details

Target: CRISPR-Cas9

Binding Specificity: C-Term

Reactivity: Streptococcus pyogenes

Host: Rabbit

Clonality: Polyclonal

Conjugate: DyLight 549

Application: Western Blotting (WB), ELISA, Fluorescence Microscopy (FM)

Purification: Anti-Cas9 antibody is directed against Streptococcus pyogenes Cas-9. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Immunogen:

Immunogen: Cas-9 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal of Streptococcus pyogenes Cas-9 protein.

Immunogen Type: Peptide

Isotype: IgG

Labeling Ratio: 4.8

Application Details

Application Notes:

Application Note: Cas9 DyLight™549 Conjugated Antibody has been tested for use in ELISA, Immunofluorescence, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band ~158 kDa in size corresponding to Cas-9 protein by western blotting in the appropriate cell lysate or extract.

ELISA Dilution: 1,25,000-1:30,000

Western Blot Dilution: 1 μg/mL

IF Microscopy Dilution: 1 μg/mL

Other Performance Data: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Target Details

Target: CRISPR-Cas9

Alternative Name: Cas 9

Background:

Synonyms: rabbit anti-Cas9 antibody DyLight™ 549 conjugation, DyLight™ 549 conjugated rabbit anti-Cas9 antibody, Cas-9, Cas 9, CRISPR, CRISPR-associated endonuclease Cas9/Csn1, SpCas9, SpyCas9, csn1, Cas-9 Antibody

Background: The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR Associated (Cas) system was discovered in bacteria as a defense against foreign DNA, even being believed to act as a sort of prokaryotic immune system. The Cas9 protein is becoming a a useful tool in the field of genomic editing for its ability to induce site-directed double strand breaks in DNA. It can cleave almost any sequence complementary to the guide RNA and has recently been used for modifying the genome of human embryos for the first time.

Gene Name: cas9

Gene ID: 901176

NCBI Accession: NP_269215

UniProt: Q99ZW2