MRGPRC antibody (N-Term)

Catalog No. ABIN6658280

Product Details anti-MRGPRC Antibody

Target: MRGPRC (MAS-Related G-Protein Coupled Receptor, Member C (MRGPRC))

Binding Specificity: N-Term

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MRGPRC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purification: Anti-MRGPRC antibody is directed against rat MRGPRC protein.  The product was affinity purified from monospecific antiserum by immunoaffinity purification.  A BLAST analysis was used to suggest reactivity with this protein from rat based on 100% homology for the immunogen sequence.  Cross reactivity with MRGPRC from other sources has not been determined.

Immunogen:

Immunogen: MRGPRC/SNSR affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a sequence at the N-terminal of rat MRGPRC.

Immunogen Type: Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:100-1:500

Application Note: Anti-MRGPRC is useful for Immunohistochemistry -P and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~37.1 kDa corresponding to the appropriate cell lysate or extract.

Western Blot Dilution: 0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide

Stabilizer: 5 mg BSA

0.05 mg Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Target Details for MRGPRC

Target: MRGPRC (MAS-Related G-Protein Coupled Receptor, Member C (MRGPRC))

Alternative Name: MRGPRC/SNSR (MRGPRC Products)

Synonyms: MrgC antibody, Snsr antibody, MAS-related G-protein coupled receptor, member C antibody, Mrgprc antibody

Background:

Synonyms: Sensory neuron-specific G-protein coupled receptor 1

Background: MAS-related G-protein coupled receptor, member C, also known as MRGPRC or MRGPRX1, is a human gene. The Mas-related genes (Mrgs) comprise a family of >50 G protein-coupled receptors (GPCRs), many of which are expressed in specific subsets of nociceptive sensory neurons in mice. In contrast, humans contain a related but nonorthologous family of genes, called MrgXs or sensory neuron-specific receptors, of which many fewer appear to be expressed in sensory neurons. This antibody is suitable for researchers interested in neuroscience research.

Gene Name: MRGPRX1

Gene ID: 282547

NCBI Accession: NP_001002286

UniProt: Q8R4G1