CaMKII alpha/beta (pThr286) antibody

Catalog No. ABIN6658207

Supplier Product No.: 612-401-c71

Product Details

Target: CaMKII alpha/beta

Binding Specificity: pThr286

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Supplier Product No.: 612-401-c71

Supplier: Rockland

Purpose: CaM Kinase II phospho T286 Antibody

Cross-Reactivity (Details): Anti-CaM Kinase II pT286 Antibody is directed against CaM Kinase II protein phosphorylated at T286.

Purification: The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

Immunogen: Anti-CaM Kinase II pT286 Antibody was produced by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Thr286.

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10,000

Western_Blot_Dilution: 1:1000

Comment:

Anti-CaM Kinase II pT286 antibody is tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 50 kDa in size corresponding to alpha CaM Kinase II and 60 kDa corresponding to beta CaM Kinase II proteins phosphorylated at Thr286.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details

Target: CaMKII alpha/beta

Alternative Name: Camk2A/B

Background:

Synonyms: Calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha, Camk2a protein

Background: CaM Kinase II antibody reacts with Ca2+/Calmodulin-Dependent Protein Kinase II (CaM Kinase II) which is a multi-functional calcium and calmodulin-dependent protein kinase that mediates cellular responses to a wide variety of intercellular signals. CaM Kinase II has been shown to regulate diverse cellular functions including synaptic plasticity, neurotransmitter synthesis and release, gene expression, ion channel function, carbohydrate metabolism, cytoskeletal function, and Ca2+-homeostasis. Phosphorylation of Thr286 on the kinase produces an autonomously active form of CaM Kinase II. Autophosphorylation of Thr305 inhibits the activity CaM Kinase II. Phosphorylation at this site appears to reduce the association of CaM Kinase II with the PSD and reduce LTP and learning. Anti-CaM Kinase II antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

Gene Name: CAMK2A/B

Gene ID: 25400

NCBI Accession: NP_037052

UniProt: P11275