GRM1a / GRM5 antibody (C-Term)

Catalog No. ABIN6658205

Product Details anti-GRM1a / GRM5 Antibody

Target: GRM1a / GRM5 (GRM5/1a) (Glutamate Receptor, Metabotropic 5/1a (GRM5/1a))

Binding Specificity: C-Term

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRM1a / GRM5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Cross-Reactivity (Details): Cross reactivity with mGluR5/1a from other species has not been determined.

Purification: Anti-mGluR5/1a antibody is directed against GluR5/1a. GluR5/1a antibody was affinity purified from monospecific antiserum by immunoaffinity purification. Immunolabeling is blocked by preadsorption of antibody with the peptide used as antigen to generate the antibody. The antibody is a total IgG fraction. Expect reactivity with the following species based on sequence homology: human, mouse, rat.

Immunogen:

Immunogen: Anti-mGluR5/1a Antibody was produced in rabbit by repeated immunizations with synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH.

Immunogen Type: Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:500

Application Note: Anti-mGluR5/1a (Rabbit) antibody is suitable for use in Western Blotting and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 125 kDa monomer and 250 kDa dimer in size corresponding to mGluR5 and mGluR1 proteins in the appropriate cell lysate or extract.

Western Blot Dilution: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Target Details for GRM1a / GRM5

Target: GRM1a / GRM5 (GRM5/1a) (Glutamate Receptor, Metabotropic 5/1a (GRM5/1a))

Alternative Name: mGluR5/1a (GRM5/1a Products)

Synonyms: mGluR5 antibody, mGlur5 antibody, glutamate metabotropic receptor 5 antibody, Grm5 antibody

Background:

Synonyms: mGluR5, Metabotropic Glutamate Receptor 5/1a

Background: mGluR5/1a antibody detects GluR5/1a protein. The metabotropic glutamate receptors (mGluRs) are key receptors in the modulation of excitatory synaptic transmission in the central nervous system. They are implicated in many forms of neural plasticity as well as learning and memory and drug abuse. Group I metabotropic glutamate receptors (consisting of mGluR1 and mGluR5) are G-protein-coupled neurotransmitter receptors that are localized in the perisynaptic region of the postsynaptic membrane. When activated, Group I mGluRs lead to stimulation of phospholipase and activation of Protein Kinase C. In contrast, activation of Group II metabotropic receptors (mGluR2 and mGluR3) leads to inhibition of adenylate cyclase. The mGluR1 receptor may also be critically involved in limiting the deleterious effects of excitotoxicity. In contrast, the mGluR5 receptor appears to be essential for late phase LTP in area CA1 of the hippocampus. mGluR5/1a is ideal for investigators involved neuronal circuitry and more generally in Neuroscience.

Gene Name: GRM5

Gene ID: 24418

UniProt: P31424