DLG4 antibody

Catalog No. ABIN6658153

Supplier Product No.: 200-301-g33

Product Details anti-DLG4 Antibody

Target: DLG4 (Discs, Large Homolog 4 (Drosophila) (DLG4))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This DLG4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Supplier Product No.: 200-301-g33

Supplier: Rockland

Purpose: PSD95 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with PSD95 from mouse, rat, and bovine based on 100 % homology with the immunizing sequence.

Purification: Anti-PSD95 Antibody was purified by Protein G chromatography.

Sterility: Sterile filtered

Immunogen: PSD95 Antibody was produced in mice by repeated immunizations raised against recombinant rat PSD-95.

Clone: 7E3

Isotype: IgG2a

Application Details

Application Notes: Optional[Neutralization_Dilution]: 1.0 μg/mL

Comment:

Anti-PSD95 Antibody is tested for WB, IHC, IF. Expect a band approximately ~100kDa protein corresponding to the molecular mass of PSD-95 on SDS PAGE immunoblots. Additional cross-reactive bands are detected at ~80kDa and ~50kDa in rat and mouse samples. Specific conditions for reactivity should be optimized by the end user. Product is provided in PBS pH 7.4.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: See application note.

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for DLG4

Target: DLG4 (Discs, Large Homolog 4 (Drosophila) (DLG4))

Alternative Name: PSD95 (DLG4 Products)

Background:

Synonyms: DLG4, SAP90, Synapse-associated protein 90, Postsynaptic density protein 95, Disks large homolog 4

Background: Postsynaptic Density protein 95 (PSD95), also known as Synapse associated protein 90 kDa, is a member of the membrane-associated guanylate kinase (MAGUK) family of proteins. PSD95 is a scaffolding protein and is involved in the assembly and function of the postsynaptic density complex. These family members consist of an N-terminal variable segment followed by three amino-terminal PDZ domains, an upstream SH3 domain and an inactive carboxyl-terminal guanylate kinase (GK) domain. The first and second PDZ domain localize NMDA receptors and K+ channels to synapses, and the third binds to neuroligins which are neuronal cell adhesion molecules that interact with b-neurexins and form intercellular junctions. PSD-95 also binds to neuronal nitric oxide synthase, possibly through interactions between PDZ domains present on both proteins. Thus different PDZ domains of PSD-95 might be specialized for distinct functions. PSD95 participates in synaptic targeting of AMPA receptors through an indirect manner involving Stargazin and related transmembrane AMPA receptor regulatory proteins (TARPs). The protein is implicated in experience dependent plasticity and plays an indispensable role in learning. Mutations in PSD95 are associated with autism.

Gene Name: Dlg4

Gene ID: 13385

NCBI Accession: NP_031890

UniProt: P31016

Pathways: Regulation of Muscle Cell Differentiation, Synaptic Membrane, Skeletal Muscle Fiber Development, Asymmetric Protein Localization, Regulation of long-term Neuronal Synaptic Plasticity