CASK antibody

Catalog No. ABIN6658140

Supplier Product No.: 200-301-f02

Product Details anti-CASK Antibody

Target: CASK (Calcium/calmodulin-Dependent serine Protein Kinase (MAGUK Family) (CASK))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CASK antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Supplier Product No.: 200-301-f02

Supplier: Rockland

Purpose: CASK Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with CASK from Mouse, Rat, Human, Zebrafish, and Xenopus based on 100 % homology with the immunizing sequence.

Purification: Anti-CASK Antibody was purified by Protein G chromatography.

Sterility: Sterile filtered

Immunogen: CASK Antibody was produced in mice by repeated immunizations with a fusion protein of rat CASK/Lin2.

Clone: S56A-50

Isotype: IgG1

Application Details

Application Notes:

Immunoprecipitation_Dilution: User Optimized

Immunohistochemistry_Dilution: User Optimized

Western_Blot_Dilution: 1:1000

Comment:

Anti-CASK Antibody is tested for use in WB and IHC. Expect a band approximately ~100kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for CASK

Target: CASK (Calcium/calmodulin-Dependent serine Protein Kinase (MAGUK Family) (CASK))

Alternative Name: CASK (CASK Products)

Background:

Synonyms: Peripheral plasma membrane protein CASK, Calcium/calmodulin-dependent serine protein kinase

Background: CASK (calmodulin sensitive kinase) is a ~112 kDa member of the membrane associated quanylate kinase (MAGUK) protein family. It is an adaptor protein with a calcium/calmodulin-dependent protein kinase domain, a SH3 domain, aguanylate kinase homology domain (GUK) and a PDZ domain. CASK links transmembrane proteins to the cytoskeleton and signaling molecules. In particular, CASK binds to Neurexin to stabilize pre- and post-synaptic structrures. While most of CASK proteins are cytoplasmic, a portion of the protein enters the nucleus, where it acts as a transcriptional co-activator. Manifesting the importance of CASK, transgenic mice with insertional mutations die within 24 hours of births. CASK is also localized in the nuclei of basal keratinocytes in newborn rodent skin and developing hair follicles. It may play a role in wound healing.

Gene Name: Cask

Gene ID: 29647

NCBI Accession: NP_071520

UniProt: Q62915

Pathways: Synaptic Vesicle Exocytosis