IP3 Receptor antibody (C-Term)

Catalog No. ABIN6658135

Product Details anti-IP3 Receptor Antibody

Target: IP3 Receptor (ITP-R83A) (Inositol 1,4,5,-Tris-Phosphate Receptor (ITP-R83A))

Binding Specificity: C-Term

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This IP3 Receptor antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Purification: Anti-IP3 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with IP3 from Human, Mouse, and Rat based on 100% homology with the immunizing sequence. Cross-reactivity with IP3 from other sources has not been determined. Ion Channels research.

Immunogen:

Immunogen: IP3 Receptor Antibody was produced in mice by repeated immunizations raised against a fusion protein of the cytoplasmic carboxyl terminus region of rat type1 IP3R inositol 1,4,5-triphosphate receptor type 1, Itpr1.

Immunogen Type: Recombinant Protein

Clone: S24-18

Isotype: IgG1

Application Details

Application Notes:

Immunohistochemistry Dilution: 0.1-1.0 μg/mL

Application Note: Anti-IP3 Antibody is suitable for use in WB, IP, and IHC. Expect a band approximately ~300 kDa on specific lysates. Does not recognize type 2 or type 3 IP3R's. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1 μg/mL

IF Microscopy Dilution: 1.0-10 μg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Target Details for IP3 Receptor

Target: IP3 Receptor (ITP-R83A) (Inositol 1,4,5,-Tris-Phosphate Receptor (ITP-R83A))

Alternative Name: IP3 Receptor (ITP-R83A Products)

Background:

Synonyms: IP3 Receptor, S24-18,Inositol 1,4,5-trisphosphate receptor type 1,IP3 receptor isoform 1, IP-3-R, IP3R1,Type 1 inositol 1,4,5-trisphosphate receptor, Insp3r

Background: Inositol 1,4,5-triphosphate receptor, also known as IP3R or InsP3R, is a member of the intracellular calcium release channel family, and is located in the endoplasmic reticulum. It functions as a Ca2+ release channel for intracellular stores of calcium ions. There are three types of IP3 receptors (IP3 receptor 1, 2 and 3) that require the second messenger inositol 1,4,5-triphosphate (IP3) for activation. Four individual receptor subunits, resulting in homo- or heterooligomerization of the receptor isoform, form a functional channel. Phosphorylation of IP3R1 at Ser1756 by cyclic- AMP-dependent protein kinase A (PKA) regulates the sensitivity of IP3R1 to IP3 and may thus be a mode of regulation for Ca2+ release. IP3R1 mediated calcium release appears to have an effect on the induction of long term depression (LTD) in Purkinje cells.

Gene Name: Itpr1

Gene ID: 25262

NCBI Accession: NP_001007236

UniProt: P29994

Pathways: Fc-epsilon Receptor Signaling Pathway, Thyroid Hormone Synthesis, Myometrial Relaxation and Contraction, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma