INA antibody

Catalog No. ABIN6658126

Product Details anti-INA Antibody

Target: INA (Internexin Neuronal Intermediate Filament Protein, alpha (INA))

Reactivity: Rat

Host: Chicken

Clonality: Polyclonal

Conjugate: This INA antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Fluorescence Microscopy (FM)

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Cross-Reactivity (Details): Cross reactivity with Alpha Internexin from other species has not been determined.

Purification: Anti-Alpha Internexin Antibody is directed against rat Alpha Internexin proteins. The antibody was purified from chicken eggs as an IgY fraction. This antibody is directed against rat alpha internexin protein. Reactivity is expected from mouse and human.

Immunogen:

Immunogen: Anti-Alpha Internexin Antibody was produced by repeated immunizations with recombinant rat alpha internexin expressed in E. coli.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Alpha Internexin antibody is suitable for use in ELISA, Western Blotting and IF. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 66 kDa in size corresponding to the alpha internexin protein in Western blots in the appropriate cell lysate or extract. Minor bands at ~150k are probably covalent dimers and bands at ~50k represent alpha-internexin breakdown products.

ELISA Dilution: 1:50,000

Western Blot Dilution: 1:5000

IF Microscopy Dilution: 1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.20.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Target Details for INA

Target: INA (Internexin Neuronal Intermediate Filament Protein, alpha (INA))

Alternative Name: Alpha Internexin (INA Products)

Synonyms: NEF5 antibody, NF-66 antibody, TXBP-1 antibody, Inexa antibody, Intlaa antibody, Nf66 antibody, AV028420 antibody, NF66 antibody, ina-A antibody, xefiltin antibody, ina-a antibody, inexa antibody, MGC82496 antibody, INA antibody, si:ch211-67n3.6 antibody, nef5 antibody, xina antibody, nf-66 antibody, txbp-1 antibody, MGC69277 antibody, gefiltin antibody, zgc:110516 antibody, internexin neuronal intermediate filament protein alpha antibody, internexin neuronal intermediate filament protein, alpha antibody, internexin neuronal intermediate filament protein alpha S homeolog antibody, low molecular weight neuronal intermediate filament S homeolog antibody, internexin neuronal intermediate filament protein, alpha a antibody, internexin neuronal intermediate filament protein, alpha b antibody, INA antibody, Ina antibody, ina.S antibody, nif.S antibody, inaa antibody, ina antibody, inab antibody

Background:

Synonyms: Alpha INX, internexin, NF-66, NEF5

Background: Anti-Alpha Internexin antibody recognizes alpha-internexin which is a Class IV intermediate filament originally discovered as it co-purifies with other neurofilament subunits. Alpha-internexin is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. It is expressed only in neurons and in large amounts early in neuronal development, but is down-regulated in many neurons as development proceeds. Many classes of mature neurons contain alpha-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons alpha-internexin is the only neurofilament subunit expressed. Antibodies to alpha-internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition, recent studies show a marked up-regulation of alpha-internexin during neuronal regeneration. The use of antibodies to this protein in the study of brain tumors has not been examined to date, but is likely to be of interest. Recently Cairns et al. used this antibody to show that alpha-internexin is an abundant component of the inclusions of neurofilament inclusion body disease (NFID), a serious human neurodegenerative disorder. The antibody was also used to confirm the presence of circulating auto-antibodies to alpha-internexin in the sera of some patients with endocrine autoimmunity, as well as in some normal individuals. Anti-Alpha Internexin antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

Gene Name: INA

Gene ID: 24503

UniProt: P23565