HCN4 antibody (C-Term)

Catalog No. ABIN6658119

Product Details anti-HCN4 Antibody

Target: HCN4 (Hyperpolarization Activated Cyclic Nucleotide-Gated Potassium Channel 4 (HCN4))

Binding Specificity: C-Term

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This HCN4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Purification: Anti-HCN4 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with HCN4 from Human, Rat, and Mouse based on 100% homology with the immunizing sequence. No cross-reactivity against other HCNs. Cross-reactivity with HCN4 from other sources has not been determined. Channels and Transporters research.

Immunogen:

Immunogen: HCN4 Antibody was produced in mice by repeated immunizations with a fusion protein corresponding to the C terminus region of rat HCN4.

Immunogen Type: Recombinant Protein

Clone: S114-10

Isotype: IgG1

Application Details

Application Notes:

Immunohistochemistry Dilution: 0.1-1.0 μg/mL

Application Note: Anti-HCN3 Antibody is suitable for use in WB, IHC and IF microscopy. Expect a band approximately ~130 kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1-10 μg/mL

IF Microscopy Dilution: 1.0-10 μg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Target Details for HCN4

Target: HCN4 (Hyperpolarization Activated Cyclic Nucleotide-Gated Potassium Channel 4 (HCN4))

Alternative Name: HCN4 (HCN4 Products)

Synonyms: SSS2 antibody, HAC4 antibody, Bcng3 antibody, Hcn3 antibody, hyperpolarization activated cyclic nucleotide gated potassium channel 4 antibody, hyperpolarization activated cyclic nucleotide-gated potassium channel 4 antibody, hyperpolarization-activated, cyclic nucleotide-gated K+ 4 antibody, HCN4 antibody, hcn4 antibody, Hcn4 antibody

Background:

Synonyms: hyperpolarization activated cyclic nucleotide gated potassium channel 4

Background: Hyperpolarization-activated cation channels of the HCN gene family contribute to spontaneous rhythmic activity in both the heart and brain.

Gene Name: Hcn4

Gene ID: 59266

NCBI Accession: NP_067690

UniProt: Q9JKA7