HCN2 antibody (C-Term)

Catalog No. ABIN6658118

Supplier Product No.: 200-301-f41

Product Details anti-HCN2 Antibody

Target: HCN2 (Hyperpolarization Activated Cyclic Nucleotide-Gated Potassium Channel 2 (HCN2))

Binding Specificity: C-Term

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This HCN2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Supplier Product No.: 200-301-f41

Supplier: Rockland

Purpose: HCN2 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with HCN2 from Rat and Mouse based on 100 % homology with the immunizing sequence.

Purification: Anti-HCN2 Antibody was purified by Protein G chromatography.

Sterility: Sterile filtered

Immunogen: HCN2 Antibody was produced in mice by repeated immunizations with a fusion protein corresponding to the cytoplasmic C-terminus region of rat HCN2.

Clone: S71

Isotype: IgG1

Application Details

Application Notes:

Immunoprecipitation_Dilution: User Optimized

Immunohistochemistry_Dilution: 1:1000

IF_Microscopy_Dilution: 1:100

Western_Blot_Dilution: 1:1000

Comment:

Anti-HCN2 Antibody is tested for use in WB, IP, ICC/IF, and IHC. Expect a band approximately ~95kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for HCN2

Target: HCN2 (Hyperpolarization Activated Cyclic Nucleotide-Gated Potassium Channel 2 (HCN2))

Alternative Name: HCN2 (HCN2 Products)

Background:

Synonyms: BCNG2, HAC1, brain cyclic nucleotide gated channel 2

Background: Hyperpolarization-activated cation channels of the HCN gene family contribute to spontaneous rhythmic activity in both the heart and brain.

Gene Name: Hcn2

Gene ID: 114244

NCBI Accession: NP_446136

UniProt: Q9JKA9