DLG1 antibody (N-Term)

Catalog No. ABIN6658116

Supplier Product No.: 200-301-g39

Product Details anti-DLG1 Antibody

Target: DLG1 (Discs, Large Homolog 1 (DLG1))

Binding Specificity: N-Term

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This DLG1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Supplier Product No.: 200-301-g39

Supplier: Rockland

Purpose: SAP97 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with SAP97 from Mouse, Human, and Rat based on 100 % homology with the immunizing sequence.

Purification: Anti-SAP97 Antibody was purified by Protein G chromatography.

Sterility: Sterile filtered

Immunogen: SAP97 Antibody was produced in mice by repeated immunizations raised against a fusion protein corresponding to an n-terminal region of rat SAP97.

Clone: S64-15

Isotype: IgG1

Application Details

Application Notes:

Immunoprecipitation_Dilution: User Optimized

Immunohistochemistry_Dilution: User Optimized

Western_Blot_Dilution: 1:1000

Comment:

Anti-SAP97 Antibody is tested for use in WB, IHC and IP. Expect a band approximately ~130kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for DLG1

Target: DLG1 (Discs, Large Homolog 1 (DLG1))

Alternative Name: SAP97 (DLG1 Products)

Background:

Synonyms: Dlgh1, SAP97, Dlg1, Synapse-associated protein 97, Disks large homolog 1

Background: Synapse-Associated Protein 97 (SAP97/human homologue of Drosophila discs-large tumor supressor or HDLG) is one of a family of plasma membrane-associated proteins found in synaptic junctions. This is a novel, presynaptic membrane protein homologous to SAP90 and the Drosophila discs-large tumor supressor protein. SAP97 has three ~90 amino acid repeats called PDZ domains, a single interior SH3 domain, and a carboxyl-terminal guanylate kinase homology (GuK) domain that is enzymatically inactive. It is hypothesized that PDZ-domain interactions play a role in receptor and channel clustering which contributes to neuronal plasticity. SAP97 is believed to participate in the clustering of certain proteins, including N-methyl-D-aspartate (NMDA) receptors and Shaker-type potassium channels at the synaptic membrane. There are two principal modes of interaction between SAP97 and other proteins. NMDA receptors and Shaker-type potassium channels both share C-terminal sequence homology consisting of a threonine/serine-X-valine-COOH (T/SXV) motif (2). Other neuronal proteins that share this motif (beta 1 adrenergic receptor, some serotonin receptors, some sodium channel subunits, and additional potassium channel subunits) may interact with SAP97 by binding to its PDZ domains. Neuronal nitric oxide synthase (nNOS), which lacks the T/SXV motif but which has its own PDZ domain, has been shown to associate with SAP97 in vitro through a pseudo-homotypic PDZ-PDZ interaction.

Gene Name: Dlg1

Gene ID: 25252

NCBI Accession: NP_036920

UniProt: Q62696

Pathways: Regulation of Actin Filament Polymerization, Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response