SHANK1 antibody (Internal Region)

Catalog No. ABIN6658104

Product Details anti-SHANK1 Antibody

Target: SHANK1 (SH3 and Multiple Ankyrin Repeat Domains 1 (SHANK1))

Binding Specificity: Internal Region

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This SHANK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Purification: Anti-SHANK1 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with SHANK1 from human, mouse, and rat based on 100% homology with the immunizing sequence. No cross-reactivity against Shank2 or Shank3. Cross-reactivity with Shank1 from other sources has not been determined. Scaffolds research.

Immunogen:

Immunogen: SHANK1 Antibody was produced in mice by repeated immunizations raised against a fusion protein corresponding to an internal region of (SH3/PDZ domains) of rat Shank1.

Immunogen Type: Recombinant Protein

Clone: S22-21

Isotype: IgG1

Application Details

Application Notes:

Immunohistochemistry Dilution: 0.1-1.0 μg/mL

Application Note: Anti-SHANK1 Antibody is suitable for use in WB and IHC. Expect a band approximately ~190-220 kDa (alternative splice variants) on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1-10 μg/mL

IF Microscopy Dilution: 1.0-10 μg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Target Details for SHANK1

Target: SHANK1 (SH3 and Multiple Ankyrin Repeat Domains 1 (SHANK1))

Alternative Name: SHANK1 (SHANK1 Products)

Synonyms: SPANK-1 antibody, SSTRIP antibody, synamon antibody, Shank1a antibody, Spank1 antibody, Sstrip antibody, SH3 and multiple ankyrin repeat domains 1 antibody, SHANK1 antibody, Shank1 antibody

Background:

Synonyms: Spank1, Sstrip, Shank1a, Shank1, SH3 and multiple ankyrin repeat domains protein 1, GKAP/SAPAP-interacting protein, Somatostatin receptor-interacting protein, SSTR-interacting protein, Synamon

Background: Shank proteins make up a family of scaffold proteins identified through their interaction with a variety of membrane and cytoplasmic proteins. Shank proteins at postsynaptic sites of excitatory synapses play roles in signal transmission into the postsynaptic neuron. Shank1 specifically might be relevant to human autism spectrum disorders, due to its differential role in specific cognitive processes, along with its importance for synapse structure and function in vivo.

Gene Name: Spank1

Gene ID: 78957

NCBI Accession: NP_113939

UniProt: Q9WV48

Pathways: Synaptic Membrane, Maintenance of Protein Location