CNTNAP1 antibody

Catalog No. ABIN6658098

Product Details anti-CNTNAP1 Antibody

Target: CNTNAP1 (Contactin Associated Protein 1 (CNTNAP1))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CNTNAP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Purification: Anti-CASPR Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with CASPR from Mouse, Human, and Rat based on 100% homology with the immunizing sequence. Cross-reactivity with CASPR from other sources has not been determined. Scaffolds research.

Immunogen:

Immunogen: CASPR Antibody was produced in mice by repeated immunizations with a fusion protein of rat CASPR.

Immunogen Type: Recombinant Protein

Clone: S65-35

Isotype: IgG1

Application Details

Application Notes:

Immunohistochemistry Dilution: 0.1-1.0 μg/mL

Application Note: Anti-CASPR Antibody is suitable for use in WB, IP, and IHC. Expect a band approximately ~220 kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Immunoprecipitation Dilution: User Optimized

Western Blot Dilution: 1 μg/mL

IF Microscopy Dilution: 1.0-10 μg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Target Details for CNTNAP1

Target: CNTNAP1 (Contactin Associated Protein 1 (CNTNAP1))

Alternative Name: CASPR (CNTNAP1 Products)

Synonyms: CNTNAP1 antibody, CASPR antibody, CNTNAP antibody, NRXN4 antibody, P190 antibody, Caspr antibody, AI841080 antibody, NCP1 antibody, Nrxn4 antibody, p190 antibody, shm antibody, contactin associated protein 1 antibody, contactin associated protein-like 1 antibody, CNTNAP1 antibody, cntnap1 antibody, Cntnap1 antibody

Background:

Synonyms: CASPR1, CNTP1, Contactin associated protein 1, NCP1, Paranodin, Neurexin 4, p190, CASPR, NRXN4

Background: Caspr (contactin-associated protein) is a part of the neurexin family. It lies in the paranodal section of the myelin sheath. It's role is for myelin sheath attachment along with contactin in a cis-complex. Caspr and Caspr2 regulate the formation of distinct axonal domains around the nodes of Ranvier. Caspr is required for the generation of a membrane barrier at the paranodal junction, whereas Caspr serves as a membrane scaffold that clusters Kv1 channels at the juxtaparanodal region. Both interact with protein 4.1B.

Gene Name: CNTNAP1

Gene ID: 8506

NCBI Accession: NP_003623

UniProt: P78357

Pathways: Cell-Cell Junction Organization