CNTNAP1 antibody

Catalog No. ABIN6658098

Supplier Product No.: 200-301-f03

Product Details anti-CNTNAP1 Antibody

Target: CNTNAP1 (Contactin Associated Protein 1 (CNTNAP1))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CNTNAP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Supplier Product No.: 200-301-f03

Supplier: Rockland

Purpose: Caspr Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with CASPR from Mouse, Human, and Rat based on 100 % homology with the immunizing sequence.

Purification: Anti-CASPR Antibody was purified by Protein G chromatography.

Sterility: Sterile filtered

Immunogen: CASPR Antibody was produced in mice by repeated immunizations with a fusion protein of rat CASPR.

Clone: S65-35

Isotype: IgG1

Application Details

Application Notes:

Immunoprecipitation_Dilution: User Optimized

Immunohistochemistry_Dilution: 0.1-1.0 μg/mL

IF_Microscopy_Dilution: 1.0-10 μg/mL

Western_Blot_Dilution: 1 μg/mL

Comment:

Anti-CASPR Antibody is tested by WB, IP, and IHC. Expect a band approximately ~220kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for CNTNAP1

Target: CNTNAP1 (Contactin Associated Protein 1 (CNTNAP1))

Alternative Name: CASPR (CNTNAP1 Products)

Background:

Synonyms: CASPR1, CNTP1, Contactin associated protein 1, NCP1, Paranodin, Neurexin 4, p190, CASPR, NRXN4

Background: Caspr (contactin-associated protein) is a part of the neurexin family. It lies in the paranodal section of the myelin sheath. It's role is for myelin sheath attachment along with contactin in a cis-complex. Caspr and Caspr2 regulate the formation of distinct axonal domains around the nodes of Ranvier. Caspr is required for the generation of a membrane barrier at the paranodal junction, whereas Caspr serves as a membrane scaffold that clusters Kv1 channels at the juxtaparanodal region. Both interact with protein 4.1B.

Gene Name: CNTNAP1

Gene ID: 84008

NCBI Accession: NP_114450

UniProt: P97846

Pathways: Cell-Cell Junction Organization