DAB1 antibody (AA 400-555)

Catalog No. ABIN6658036

Supplier Product No.: 100-401-225s

Product Details anti-DAB1 Antibody

Target: DAB1 (Disabled Homolog 1 (Drosophila) (DAB1))

Binding Specificity: AA 400-555

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DAB1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Supplier Product No.: 100-401-225s

Supplier: Rockland

Purpose: Dab1 Antibody

Cross-Reactivity (Details): This antibody is directed against Dab1 from mouse.

Purification: Dab-1 whole rabbit antiserum was prepared by delipidation and defibrination followed by the addition of buffer salts and preservative.

Sterility: Sterile filtered

Immunogen: This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to the C-terminal region of murine Dab1 at amino acids 400-555.

Isotype: IgG

Application Details

Application Notes:

Immunoprecipitation_Dilution: 1 μL per 500 μg

ELISA_Dilution: 1:10,000 - 1:50,000

Immunohistochemistry_Dilution: 1:5,000

IF_Microscopy_Dilution: User Optimized

Western_Blot_Dilution: 1:5,000

Other: User Optimized

Comment:

Suggested Applications: Biochemical Assay, IF, Multiplex
Anti-Dab1 antibody has been tested by western blot and immunohistochemistry and is suitable for the detection of Dab1 by immunoprecipitation.  Specific conditions for reactivity should be optimized by the end user.  Expect a band at 80 kDa corresponding to Dab1 in the appropriate tissue extract or cell lysate.  For western blotting block the blot using 5% BLOTTO for 1 h at room temperature and followed by incubation with the primary antibody diluted in 1% BLOTTO in TTBS for 1 h at room temperature.  For immunoprecipitation use 1 μl of antiserum per 500 μg of brain lysate.  Perform immunoprecipitation at 4°C for 2 h.  For immunoprecipitation buffer lysates with 50 mM Tris-Cl, pH 7.4, supplemented with 150 mM sodium chloride, 1% (v/v) NP-40, 10 μg/ml aprotinin and 10 μg/ml leupeptin.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

References for anti-DAB1 antibody (ABIN6658036)

Katyal, Gao, Monckton, Glubrecht, Godbout: "Hierarchical disabled-1 tyrosine phosphorylation in Src family kinase activation and neurite formation." in: Journal of molecular biology, Vol. 368, Issue 2, pp. 349-64, (2007) (PubMed).

Target Details for DAB1

Target: DAB1 (Disabled Homolog 1 (Drosophila) (DAB1))

Alternative Name: Dab1 (DAB1 Products)

Background:

Synonyms: rabbit anti-Dab 1 antibody, Disabled homolog 1 antibody, Disabled homolog 1 Drosophila antibody, Scm antibody, Scr antibody, Scrambler antibody, Yot antibody, Yotari antibody

Background: Anti-Dab1 Antibody recognizes Dab1 that is a phosphoprotein encoded by the mouse gene dab and is related to the Drosophila gene 'disabled'. Mutations in the mouse dab gene may result in the 'scrambler' and 'yotari' phenotypes. Dab1 binds to non-receptor tyrosine kinases and plays an important role in brain development. Dab1 is expressed in neuronal populations exposed to reelin, and it functions as a signaling molecule that regulates cell positioning in the developing brain. Cloning of human Dab1 and sequence determinations suggest a 96 % identity to the mouse sequence. Dab1 binds to cytoplasmic regions of very low density lipoprotein receptors (VLDLR), apolipoprotein E receptor-2 (ApoER2) and the Amyloid Precursor Protein (APP) family of proteins. Dab1 accumulates in ectopic neurons from mice lacking Reelin or both VLDLR and ApoER2. In humans, Dab1 has been mapped to lp32-p312. This region shows homology of synteny with the segment of mouse chromosome 4 containing Dab1.

Gene Name: DAB1

Gene ID: 13131

NCBI Accession: NP_034144

UniProt: P97318