SIX3 antibody (Internal Region)

Catalog No. ABIN6657913

Supplier Product No.: 600-401-a26s

Product Details anti-SIX3 Antibody

Target: SIX3 (Sine Oculis-Related Homeobox 3 (SIX3))

Binding Specificity: Internal Region

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIX3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Supplier Product No.: 600-401-a26s

Supplier: Rockland

Purpose: Six3 Antibody

Cross-Reactivity (Details): This antibody reacts with mouse Six3.

Purification: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse Six3 protein.

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10,000-1:15,000

Immunohistochemistry_Dilution: 1:100-1:250

IF_Microscopy_Dilution: 1:200 - 1:1,000

Western_Blot_Dilution: 1:500 - 1:2,000

Other: User Optimized

Comment:

Suggested Applications: IF, Multiplex
This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 37 kDa in size corresponding to Six3 by western blotting in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 3 months

References for anti-SIX3 antibody (ABIN6657913)

Brignani, Raj, Schmidt, Düdükcü, Adolfs, De Ruiter, Rybiczka-Tesulov, Verhagen, van der Meer, Broekhoven, Moreno-Bravo, Grossouw, Dumontier, Cloutier, Chédotal, Pasterkamp: "Remotely Produced and Axon-Derived Netrin-1 Instructs GABAergic Neuron Migration and Dopaminergic Substantia Nigra Development." in: Neuron, Vol. 107, Issue 4, pp. 684-702.e9, (2020) (PubMed).

Roy, Murphy, Deng, MacDonald, Bammler, Aldinger, Glass, Millen: "PI3K-Yap activity drives cortical gyrification and hydrocephalus in mice." in: eLife, Vol. 8, (2020) (PubMed).

Li, Gao, Wang, Song, Xu, Xie, Zhou, Pan, Peng, Zhang, Ge, Zhong: "Generation and Characterization of Induced Pluripotent Stem Cells and Retinal Organoids From a Leber's Congenital Amaurosis Patient With Novel RPE65 Mutations." in: Frontiers in molecular neuroscience, Vol. 12, pp. 212, (2019) (PubMed).

Xu, Liang, Song, Zhang, Lindtner, Li, Wen, Liu, Guo, Qi, Wang, Wang, Li, You, Wang, Chen, Feng, Rubenstein, Yang: "SP8 and SP9 coordinately promote D2-type medium spiny neuron production by activating Six3 expression." in: Development (Cambridge, England), Vol. 145, Issue 14, (2018) (PubMed).

Li, Xie, He, Zhou, Gao, Liu, Pan, Ge, Peng, Zhong: "Generation of Retinal Organoids with Mature Rods and Cones from Urine-Derived Human Induced Pluripotent Stem Cells." in: Stem cells international, Vol. 2018, pp. 4968658, (2018) (PubMed).

Diacou, Zhao, Zheng, Cvekl, Liu: "Six3 and Six6 Are Jointly Required for the Maintenance of Multipotent Retinal Progenitors through Both Positive and Negative Regulation." in: Cell reports, Vol. 25, Issue 9, pp. 2510-2523.e4, (2018) (PubMed).

Target Details for SIX3

Target: SIX3 (Sine Oculis-Related Homeobox 3 (SIX3))

Alternative Name: Six3 (SIX3 Products)

Background:

Synonyms: rabbit anti-Six3 antibody, Six-3, Six 3, Homeobox protein SIX3, Sine oculis homeobox homolog 3

Background: Six3 (also known as sine oculis homeobox homolog 3) is involved in the development of the visual system and forebrain.  Six3 is a nuclear protein that is reported to exist in two forms by alternative splicing of the gene product.  Six3 is first expressed at E6.5 of mouse embryonic development around the anterior border. At E8.5, expression is found over the anterior neural plate. At E9.5, it is in the diencephalic part of the ventral forebrain, optic vesicles, olfactory placodes and Rathke's pouch. In later stages, Six3 is present in hypothalamus, eyes and pituitary.

Gene Name: Six3

Gene ID: 20473, 59939908

UniProt: Q62233

Pathways: Protein targeting to Nucleus