SIX3 antibody (Internal Region)
-
- Target See all SIX3 Antibodies
- SIX3 (Sine Oculis-Related Homeobox 3 (SIX3))
-
Binding Specificity
- Internal Region
-
Reactivity
- Mouse
-
Host
-
Guinea Pig
-
Clonality
- Polyclonal
-
Conjugate
- This SIX3 antibody is un-conjugated
-
Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM)
- Purification
- This product was purified by Protein A chromatography from monospecific antiserum. This antibody reacts with mouse Six3. Expect partial reactivity with Six3 from chicken and Xenopus sources based on 95% and 85% homologies, respectively. Cross-reactivity with Six3 from other sources has not been determined.
- Immunogen
-
Immunogen: This Protein A purified antibody was prepared from whole guinea pig serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse Six3 protein.
Immunogen Type: Peptide
- Isotype
- IgG
- Top Product
- Discover our top product SIX3 Primary Antibody
-
-
- Application Notes
-
Immunohistochemistry Dilution: 1:200
Application Note: This Protein A purified antibody has been tested for use in ELISA, immunofluorescence microscopy and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 37 kDa in size corresponding to Six3 by western blotting in the appropriate cell lysate or extract.
ELISA Dilution: 1:5,000 - 1:25,000
Western Blot Dilution: 1:1000
IF Microscopy Dilution: 1:200 - 1:1,000
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Buffer
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01 % (w/v) Sodium AzideStabilizer: None
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
-
-
TCF7L2 regulates postmitotic differentiation programmes and excitability patterns in the thalamus." in: Development (Cambridge, England), Vol. 147, Issue 16, (2021) (PubMed).
: "Transcriptional regulatory control of mammalian nephron progenitors revealed by multi-factor cistromic analysis and genetic studies." in: PLoS genetics, Vol. 14, Issue 1, pp. e1007181, (2018) (PubMed).
: "
-
TCF7L2 regulates postmitotic differentiation programmes and excitability patterns in the thalamus." in: Development (Cambridge, England), Vol. 147, Issue 16, (2021) (PubMed).
-
- Target
- SIX3 (Sine Oculis-Related Homeobox 3 (SIX3))
- Alternative Name
- SIX3 (SIX3 Products)
- Synonyms
- HPE2 antibody, SIX3 antibody, cb347 antibody, six3.2 antibody, six6 antibody, E130112M24Rik antibody, Six3a antibody, Six3alpha antibody, Six3b antibody, Six3beta antibody, six3 antibody, six3.1 antibody, wu:fc10a08 antibody, SIX homeobox 3 antibody, SIX homeobox 3b antibody, sine oculis-related homeobox 3 antibody, SIX homeobox 3a antibody, sine oculis homeobox homolog 3 antibody, SIX3 antibody, six3b antibody, Six3 antibody, six3a antibody, six3 antibody
- Background
-
Synonyms: guinea pig anti-SIX 3 antibody, Six3, Sine oculis homeobox homolog 3
Background: Six3 (also known as sine oculis homeobox homolog 3) is involved in the development of the visual system and forebrain. Six3 is a nuclear protein that is reported to exist in two forms by alternative splicing of the gene product. Six3 is first expressed at E6.5 of mouse embryonic development around the anterior border. At E8.5, expression is found over the anterior neural plate. At E9.5, it is in the diencephalic part of the ventral forebrain, optic vesicles, olfactory placodes and Rathke's pouch. In later stages, Six3 is present in hypothalamus, eyes and pituitary.
Gene Name: SIX3
- Gene ID
- 20473, 59939908
- UniProt
- Q62233
- Pathways
- Protein targeting to Nucleus
-