TCRb antibody (FITC)

Catalog No. ABIN6657906

Supplier Product No.: 200-b02-n92

Product Details anti-TCRb Antibody (FITC)

Target: TCRb (T Cell Receptor beta Chain (TCRb))

Reactivity: Mouse

Host: Hamster

Clonality: Monoclonal

Conjugate: This TCRb antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Supplier Product No.: 200-b02-n92

Supplier: Rockland

Purpose: TCRbeta Fluorescein Antibody

Cross-Reactivity (Details): Cross reactivity with TCRbeta from other sources has not been tested.

Purification: Fluorescein conjugated TCRbeta Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse TCRbeta.

Sterility: Sterile filtered

Immunogen: Anti-TCRbeta Antibody (Monoclonal) was produced by repeated immunizations with TCRbeta antigen.

Clone: H57-597

Isotype: IgG

Labeling Ratio: 2-8

Application Details

Application Notes:

Immunoprecipitation_Dilution: User Optimized

Flow_Cytometry_Dilution: 10 μL/10^6 cells (0.1 μg)

IF_Microscopy_Dilution: User Optimized

Other: Sufficient to run approximately 500 tests

Comment:

Anti-TCR beta is tested for FLOW and useful for Immunofluorescence and Immunoprecipitation using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.1 % Gelatin

Preservative: 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  DO NOT FREEZE. This product is light sensitive.

Expiry Date: 6 months

Target Details for TCRb

Target: TCRb (T Cell Receptor beta Chain (TCRb))

Alternative Name: Tcrb (TCRb Products)

Background:

Synonyms: Tib, TCRbeta, T cell receptor beta chain, Tcrb

Background: The H57-597 antibody is specific for the beta chain of the mouse T cell Receptor (TCR). This cell surface protein combines with a second protein chain (alpha chain) to form the alpha-beta TCR that is expressed by NK1.1+ thymocytes, NKT cells, and the majority of peripheral T cells. A small number of T cells may express an alternative heteromer of gamma and delta protein chains, known as the gamma-delta TCR. These receptors participate in a complex with CD3, and with the co-receptors CD4 or CD8, to recognize and respond to antigens bound to MHC molecules on antigen-presenting cells. Such interactions promote T cell receptor signaling (T cell activation) and can result in a number of cellular responses including proliferation, differentiation, production of cytokines or activation-induced cell death.The H57-597 antibody is used as a phenotypic marker for T cells expressing the alpha-beta TCR. It is also widely used to cross-link surface TCR and thereby mimic TCR-mediated cell activation or induction of apoptosis. The antibody does not cross-react with cells expressing the gamma-delta TCR.

Gene Name: Tcrb

Gene ID: 21577