KDM5B antibody (Internal Region)

Catalog No. ABIN6657904

Supplier Product No.: 100-401-v92

Product Details anti-KDM5B Antibody

Target: KDM5B (Lysine (K)-Specific Demethylase 5B (KDM5B))

Binding Specificity: Internal Region

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KDM5B antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Supplier Product No.: 100-401-v92

Supplier: Rockland

Purpose: Jarid1b Antibody

Cross-Reactivity (Details): Cross reactivity with Jarid1b from other species not tested.

Purification: Anti-Jarid1b Antibody is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration.

Sterility: Sterile filtered

Immunogen: Anti-Jarid1b Antibody was produced in rabbits by repeated immunizations with a synthetic peptide containing an amino acid sequence from the internal part of mouse Jarid1b.

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:100 - 1:200

Western_Blot_Dilution: 1:500

Comment:

Anti-Jarid1b Antibody has been tested by ELISA and Western Blots. Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 175 kDa in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: None

Stabilizer: None

Preservative: 0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for KDM5B

Target: KDM5B (Lysine (K)-Specific Demethylase 5B (KDM5B))

Alternative Name: Jarid1b (KDM5B Products)

Background:

Synonyms: Lysine-specific demethylase 5B, Histone demethylase JARID1B, Jumonji/ARID domain-containing protein 1B, PLU-1

Background: JARID1B is a histone demethylase which specifically demethylates mono-, di, and trimethylated lysine 4 of histone H3. It acts as a transcriptional repressor for the tumor suppressor genes BRCA1 and HOXA5 and has been associated with breast cancer development. In contrast, in melanoma JARID1B itself may function as a tumor suppressor. Anti-Jarid1b Antibody is ideal for research in Epigenetics, Chromatin Remodeling and Cancer.

Gene Name: Kdm5b

Gene ID: 10765

NCBI Accession: NP_006609

UniProt: Q80Y84

Pathways: Warburg Effect