CD207 antibody

Catalog No. ABIN6657890

Supplier Product No.: 200-401-h89

Product Details anti-CD207 Antibody

Target: CD207 (CD207 Molecule, Langerin (CD207))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD207 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Supplier Product No.: 200-401-h89

Supplier: Rockland

Purpose: Langerin Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with Anti-Langerin from mouse based on 100 % homology with the immunizing sequence.

Purification: Anti-Langerin Antibody was purified by Protein G chromatography.

Immunogen: Langerin Antibody was produced from whole rabbit serum prepared by repeated immunizations with mouse Langerin protein at an internal amino acid sequence.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry_Dilution: 5 μg/mL (paraffin)

Western_Blot_Dilution: 1-3 μg/mL

Comment:

Anti-Langerin Antibody is tested for use in WB, Flow, Flow-IC, IHC, IHC-Fr, and IHC-P. Expect a band approximately 36kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 ,0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for CD207

Target: CD207 (CD207 Molecule, Langerin (CD207))

Alternative Name: Langerin (CD207 Products)

Background:

Synonyms: Clec4k, C-type lectin domain family 4 member K, Langerin, CD207

Background: Anti-Langerin Antibody detects mouse Langerin. Langerin (CD207) is a type II membrane-associated C-type lectin known to be expressed exclusively by Langerhans cells. Langerin (CD207) recognizes mannose residues via its single carbohydrate recognition domain (CRD). Langerin (CD207) is localized not only on the cell surface, but also intracellularly in close association with Birbeck granules. Transfection of Langerin cDNA into fibroblasts creates a compact network of membrane structures with typical features of Birbeck granules (BG). Langerin (CD207) is thus a potent inducer of membrane superimposition and zippering leading to BG formation. Anti-Langerin Antibody is ideal for investigators involved in Toll Like Receptors, signaling, and extracelluar matrix research.

Gene Name: Cd207

Gene ID: 246278

NCBI Accession: NP_659192

UniProt: Q8VBX4