CD19 antibody (PE)

Catalog No. ABIN6657888

Supplier Product No.: 200-508-m72

Product Details anti-CD19 Antibody (PE)

Target: CD19 (CD19 Molecule (CD19))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD19 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Supplier Product No.: 200-508-m72

Supplier: Rockland

Purpose: CD19 Phycoerythrin Antibody

Cross-Reactivity (Details): Reactivity is observed against mouse CD19.

Purification: Phycoerythrin conjugated CD19 Monoclonal Antibody was Protein G Purified from tissue culture supernatant and is directed against mouse CD19.

Sterility: Sterile filtered

Immunogen: Anti-CD19 Antibody (Monoclonal) was produced by repeated immunizations with mouse CD19 transfected cell line.

Clone: 1D3

Isotype: IgG2a kappa

Labeling Ratio: 1-2

Application Details

Application Notes: Optional[Neutralization_Dilution]: 0.25-1 μg/10^6 cells

Comment:

Anti-CD19 is tested for Flow Cytometry (Cell Surface). Researchers should determine optimal titers for applications that are not stated.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.1 % Gelatin

Preservative: 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Expiry Date: 6 months

Target Details for CD19

Target: CD19 (CD19 Molecule (CD19))

Alternative Name: CD19 (CD19 Products)

Background:

Synonyms: B-lymphocyte antigen CD19, Differentiation antigen CD19, CD19

Background: CD19 is a member of the immunoglobulin superfamily with 556 amino acid and two immunoglobulin-like C2-type domains. As a cell surface protein, CD19 is known to form a complex with CD21, CD81 and CD225 in the membrane of mature B cells. A major function of CD100 is to assemble with the antigen receptor of B lymphocytes so as to decrease the threshold for antigen receptor dependent stimulation thus enhancing the specificity and sensitivity of B cells towards antigens. Thus CD19 is an important protein for B cell antigen receptor signaling and regulation and also acts as a specialized adaptor protein for the amplification of Src family needed for this purpose. Regulation of growth of B cells is a major function of CD19 and its expression is confined to only B lymphocytes and follicular dendritic cells of the hematopoietic system. Increased expression of CD19 induces the production of auto-antibody thus giving an insight to the regulatory role of CD19 in autoimmunity. Defects in CD19 are a cause of hypogammaglobulinemia.

Gene Name: Cd19

Gene ID: 12478

NCBI Accession: NP_033974

UniProt: P25918

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway