ASH2L antibody (C-Term)

Catalog No. ABIN6657882

Product Details anti-ASH2L Antibody

Target: ASH2L (Set1/Ash2 Histone Methyltransferase Complex Subunit ASH2 (ASH2L))

Binding Specificity: C-Term

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ASH2L antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Fluorescence Microscopy (FM)

Purpose: Ash2 Antibody

Cross-Reactivity (Details): Cross reactivity with Ash2 from other species not tested.

Purification: Anti-Ash2 Antibody is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration.

Sterility: Sterile filtered

Immunogen: Anti-Ash2 Antibody was produced in rabbits by repeated immunizations with three different synthetic peptides of mouse Ash2: two containing an amino acid sequence from the internal and one containing an amino acid sequence from the C-terminal part of the protein.

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:100 - 1:500

IF_Microscopy_Dilution: 1:200

Western_Blot_Dilution: 1:1,000

Comment:

Anti-Ash2 Antibody has been tested by ELISA, Immunofluorescence and Western Blots. Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 68 kDa in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: None

Stabilizer: None

Preservative: 0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for ASH2L

Target: ASH2L (Set1/Ash2 Histone Methyltransferase Complex Subunit ASH2 (ASH2L))

Alternative Name: ASH2L (ASH2L Products)

Synonyms: ASH2 antibody, ASH2L1 antibody, ASH2L2 antibody, Bre2 antibody, fb34c06 antibody, fe38b04 antibody, fi20h02 antibody, wu:fb34c06 antibody, wu:fe38b04 antibody, wu:fi20h02 antibody, zgc:171444 antibody, ASH2L antibody, ash2-like antibody, ASH2 like histone lysine methyltransferase complex subunit antibody, ash2 (absent, small, or homeotic)-like (Drosophila) antibody, ash2 (absent, small, or homeotic)-like L homeolog antibody, ASH2L antibody, Ash2l antibody, ash2l antibody, ash2l.L antibody

Background:

Synonyms: Set1/Ash2 histone methyltransferase complex subunit ASH2, ASH2-like protein, ASH2L1, ASH2L2, Bre2

Background: Ash2 is a component of the Set1/Ash2 histone methyltransferase (HMT) complex. This complex specifically methylates K4 of histone H3, thereby activating transcription. Methylation of K4 is blocked by premethylation of the neighboring K9, a repressor of transcription. This indicates that the Set1/Ash2 HMT complex mediates the crosstalk between K9 methylation and K4 methylation. Ash2 plays a role in hematopoiesis and may be associated with some kinds of leukemia. Anti-Ash2 Antibody is ideal for research in Gene Expression, Epigenetics, Cell Signaling and Cancer.

Gene Name: ASH2L

Gene ID: 9070

NCBI Accession: NP_001098684

UniProt: Q9UBL3