ASCL2 antibody (C-Term)

Catalog No. ABIN6657882

Product Details anti-ASCL2 Antibody

Target: ASCL2 (Achaete-Scute Complex Homolog 2 (ASCL2))

Binding Specificity: C-Term

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ASCL2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Fluorescence Microscopy (FM)

Cross-Reactivity (Details): Cross reactivity with Ash2 from other species not tested.

Purification: Anti-Ash2 Antibody is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration.

Immunogen:

Immunogen: Anti-Ash2 Antibody was produced in rabbits by repeated immunizations with three different synthetic peptides of mouse Ash2: two containing an amino acid sequence from the internal and one containing an amino acid sequence from the C-terminal part of the protein.

Immunogen Type: Peptide

Application Details

Application Notes:

Application Note: Anti-Ash2 Antibody is suitable for ELISA, Immunofluorescence and Western Blots. Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 68 kDa in the appropriate cell lysate or extract.

ELISA Dilution: 1:100 - 1:500

Western Blot Dilution: 1:1,000

IF Microscopy Dilution: 1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

0.01 % (w/v) Sodium Azide

Stabilizer: None

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Target Details for ASCL2

Target: ASCL2 (Achaete-Scute Complex Homolog 2 (ASCL2))

Alternative Name: Ash2 (ASCL2 Products)

Synonyms: ASH2 antibody, HASH2 antibody, MASH2 antibody, bHLHa45 antibody, ASCL2 antibody, 2410083I15Rik antibody, Mash2 antibody, achaete-scute family bHLH transcription factor 2 antibody, ASCL2 antibody, Ascl2 antibody

Background:

Synonyms: Set1/Ash2 histone methyltransferase complex subunit ASH2, ASH2-like protein, ASH2L1, ASH2L2, Bre2

Background: Ash2 is a component of the Set1/Ash2 histone methyltransferase (HMT) complex. This complex specifically methylates K4 of histone H3, thereby activating transcription. Methylation of K4 is blocked by premethylation of the neighboring K9, a repressor of transcription. This indicates that the Set1/Ash2 HMT complex mediates the crosstalk between K9 methylation and K4 methylation. Ash2 plays a role in hematopoiesis and may be associated with some kinds of leukemia. Anti-Ash2 Antibody is ideal for research in Gene Expression, Epigenetics, Cell Signaling and Cancer.

Gene Name: ASH2L

Gene ID: 9070

NCBI Accession: NP_001098684

UniProt: Q9UBL3

Pathways: Stem Cell Maintenance