NK-1.1/CD161c antibody (APC)

Catalog No. ABIN6657869

Supplier Product No.: 200-326-n93

Product Details anti-NK-1.1/CD161c Antibody (APC)

Target: NK-1.1/CD161c (NK-1.1)

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This NK-1.1/CD161c antibody is conjugated to APC

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Supplier Product No.: 200-326-n93

Supplier: Rockland

Purpose: NK1.1 Allophycocyanin Antibody

Purification: Allophycocyanin conjugated NK1.1 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse NK1.

Sterility: Sterile filtered

Immunogen: Anti-NK1.1 Antibody (Monoclonal) was produced by repeated immunizations with NK1.1 antigen.

Clone: PK136

Isotype: IgG2a kappa

Labeling Ratio: 1-2

Application Details

Application Notes:

Immunoprecipitation_Dilution: User Optimized

Immunohistochemistry_Dilution: User Optimized

Flow_Cytometry_Dilution: 10 μL/10^6 cells (0.1 μg)

IF_Microscopy_Dilution: User Optimized

Comment:

Anti-NK1.1 is tested in Flow Cytometry useful for Immunohistochemistry, Immunoprecipitation, Immunofluorescence using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.1 % Gelatin

Preservative: 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Expiry Date: 6 months

Target Details for NK-1.1/CD161c

Target: NK-1.1/CD161c (NK-1.1)

Alternative Name: NK1.1 (NK-1.1/CD161c Products)

Background:

Synonyms: Killer cell lectin-like receptor subfamily B member 1C, CD161 antigen-like family member C, Lymphocyte antigen 55c, Ly-55c, NK1.1, NKR-P1.9, NKR-P1C, Natural killer cell surface protein P1-40, NKR-P1 40, CD161c, Klrb1c, Ly55c, Nkrp1c

Background: The PK136 antibody is specific for mouse NK1.1, a type II transmembrane lectin-like receptor and member of the killer cell lectin-like receptor (KLR) family. NK1.1 is prominently expressed on natural killer (NK) cells, and is correlated with NK cytotoxic effects toward virus-infected cells and tumor cells. NK1.1 is expressed on subsets of NKT cells in certain mouse strains (C57BL/6, FVB/N, and NZB), yet absent from others (AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129). Putative subsets of NK cells and their expression of NK1.1 antigen are of continuing interest, including NK1.1+/CD117+ (c-Kit) cells reported to be immunosuppressive for CD8+ T cells in a mechanism involving PD-1 and PD-L1. The PK136 antibody may be used for detection of NK1.1 expression on mouse strains including CE, B6, NZB, C58, Ma/My, ST, SJL, and FVB. The antibody is reported to react with an epitope common to NKR-P1B and NKR-P1C alloantigenic forms of NK1.1.

Gene Name: Klrb1c

Gene ID: 17059

NCBI Accession: NP_001153376

UniProt: P27814