CD3 epsilon antibody (FITC)

Catalog No. ABIN6657857

Supplier Product No.: 200-b02-m69

Product Details anti-CD3 epsilon Antibody (FITC)

Target: CD3 epsilon (CD3E)

Reactivity: Mouse

Host: Hamster

Clonality: Monoclonal

Conjugate: This CD3 epsilon antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Supplier Product No.: 200-b02-m69

Supplier: Rockland

Purpose: CD3e Fluorescein Antibody

Cross-Reactivity (Details): Reactivity is observed against mouse CD3.

Purification: Fluorescein conjugated CD3 Monoclonal Antibody was Protein G Purified from tissue culture supernatant and is directed against mouse CD3.

Sterility: Sterile filtered

Immunogen: Anti-CD3 Antibody (Monoclonal) was produced by repeated immunizations with H-2Kb specific cytotoxic T lymphocyte clone BM10-37.

Clone: 145-2C11

Isotype: IgG

Labeling Ratio: 2-8

Application Details

Application Notes:

Flow_Cytometry_Dilution: 0.1-0.5 μg/10^6 cells

Other: Sufficient to run approximately 500 tests

Comment:

Anti-CD3 is tested for Flow Cytometry (Cell Surface). Researchers should determine optimal titers for applications that are not stated.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.1 % Gelatin

Preservative: 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  DO NOT FREEZE. This product is light sensitive.

Expiry Date: 6 months

Target Details for CD3 epsilon

Target: CD3 epsilon (CD3E)

Alternative Name: CD3e (CD3E Products)

Background:

Synonyms: T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e

Background: CD3 is a multi-subunit complex consisting of 4 different invariable membrane proteins, CD3E, CD3D, CD3G and CD3-omega. These CD3 proteins are expressed on the surface of T-cells and Thymocytes during thymocyte development. They are non-covalently associated with the TCR heterodimer and form the functional TCR-CD3 antigen receptor complex of the T-lymphocyte. CD3 expression is significant during Intrathymic T-lymphocyte maturation, TCR signaling and Thymocyte differentiation. Stimulation of TCR-CD3 complex results in activation of various protein tyrosine kinases including ZAP-70 affecting multiple cellular responses including T-cell proliferation, clonal expansion, induction of tolerance and apoptosis. Mutations in the sub-unit chains of CD3 have been associated with various immunodeficiency syndromes including SCID.

Gene Name: Cd3e

Gene ID: 12501

NCBI Accession: NP_031674

UniProt: P22646

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway