MCL-1 antibody (Internal Region)

Catalog No. ABIN6657787

Supplier Product No.: 600-401-394s

Product Details anti-MCL-1 Antibody

Target: MCL-1 (MCL1) (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))

Binding Specificity: Internal Region

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCL-1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Supplier Product No.: 600-401-394s

Supplier: Rockland

Purpose: Mcl-1 Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.

Purification: Anti-Mcl-1 affinity purified antibody was prepared from monospecific antiserum by immunoaffinity chromatography using synthetic peptide coupled to agarose beads followed by cross adsorption to remove any unwanted reactivity.

Sterility: Sterile filtered

Immunogen: This affinity purified antibody was purified from whole rabbit serum prepared by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse Mcl-1 conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10,000 - 1:50,000

Western_Blot_Dilution: 1:10,000

Other: User Optimized

Comment:

Suggested Applications: FC, IF, IHC, IP
Anti-Mcl-1 Antibody has been tested by ELISA and western blot and is suitable for immunoprecipitation.  This antibody detects mouse Mcl-1 and is not expected to cross react with the human sequence.  Cross reactivity with Mcl-1 from other sources is unknown.  This antibody is highly specific, showing no bands in null cell lysates from Mcl-1 knockout mice even when grossly over-exposed. Mouse Mcl-1 (myeloid cell leukemia sequence 1) is composed of 331 amino acids and is reported to be 35.2 kDa in size.  The human ortholog consists of 351 amino acids and is reported to be 37.3 kDa in size.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

References for anti-MCL-1 antibody (ABIN6657787)

Karbon, Schuler, Braun, Eichin, Haschka, Drach, Sotillo, Geley, Spierings, Tijhuis, Foijer, Villunger: "Chronic spindle assembly checkpoint activation causes myelosuppression and gastrointestinal atrophy." in: EMBO reports, Vol. 25, Issue 6, pp. 2743-2772, (2024) (PubMed).

Lindenboim, Zohar, Gundersen, Worman, Stein: "LINC complex protein nesprin-2 has pro-apoptotic activity via Bcl-2 family proteins." in: Cell death discovery, Vol. 10, Issue 1, pp. 29, (2024) (PubMed).

Prucsi, Zimny, Płonczyńska, Zubrzycka, Potempa, Sochalska: "Porphyromonas gingivalis Peptidyl Arginine Deiminase (PPAD) in the Context of the Feed-Forward Loop of Inflammation in Periodontitis." in: International journal of molecular sciences, Vol. 24, Issue 16, (2023) (PubMed).

Quarato, Mari, Barrows, Yang, Ruehl, Chen, Guy, Low, Chen, Green: "Mitophagy restricts BAX/BAK-independent, Parkin-mediated apoptosis." in: Science advances, Vol. 9, Issue 21, pp. eadg8156, (2023) (PubMed).

Huang, Chin, Reljic, Djajawi, Tan, Gong, Stroud, Huang, van Delft, Dewson: "Mitochondrial E3 ubiquitin ligase MARCHF5 controls BAK apoptotic activity independently of BH3-only proteins." in: Cell death and differentiation, Vol. 30, Issue 3, pp. 632-646, (2023) (PubMed).

Pileggi, Parmar, Elkhatib, Stewart, Alecu, Côté, Bennett, Sandhu, Cuperlovic-Culf, Harper: "The SARS-CoV-2 spike glycoprotein interacts with MAO-B and impairs mitochondrial energetics." in: Current research in neurobiology, Vol. 5, pp. 100112, (2023) (PubMed).

Prew, Adhikary, Choi, Portero, Paulo, Gowda, Budhraja, Opferman, Gygi, Danial, Walensky: "MCL-1 is a master regulator of cancer dependency on fatty acid oxidation." in: Cell reports, Vol. 41, Issue 1, pp. 111445, (2022) (PubMed).

Quarato, Llambi, Guy, Min, Actis, Sun, Narina, Pruett-Miller, Peng, Rankovic, Green: "Ca2+-mediated mitochondrial inner membrane permeabilization induces cell death independently of Bax and Bak." in: Cell death and differentiation, Vol. 29, Issue 7, pp. 1318-1334, (2022) (PubMed).

Moyzis, Lally, Liang, Najor, Gustafsson: "Mcl-1 Differentially Regulates Autophagy in Response to Changes in Energy Status and Mitochondrial Damage." in: Cells, Vol. 11, Issue 9, (2022) (PubMed).

Buitrago-Molina, Marhenke, Becker, Geffers, Itzel, Teufel, Jaeschke, Lechel, Unger, Markovic, Sharma, Marquardt, Saborowski, Saborowski, Vogel: "p53-Independent Induction of p21 Fails to Control Regeneration and Hepatocarcinogenesis in a Murine Liver Injury Model." in: Cellular and molecular gastroenterology and hepatology, Vol. 11, Issue 5, pp. 1387-1404, (2022) (PubMed).

Smith, Budhraja, Lynch, Roberts, Panetta, Connelly, Turnis, Pruett-Miller, Schuetz, Mullighan, Opferman: "The Heme-Regulated Inhibitor Pathway Modulates Susceptibility of Poor Prognosis B-Lineage Acute Leukemia to BH3-Mimetics." in: Molecular cancer research : MCR, Vol. 19, Issue 4, pp. 636-650, (2022) (PubMed).

Teh, Robbins, Henstridge, Dewson, Diepstraten, Kelly, Febbraio, Gabriel, O'Reilly, Strasser, Gray: "MCL-1 is essential for survival but dispensable for metabolic fitness of FOXP3+ regulatory T cells." in: Cell death and differentiation, Vol. 27, Issue 12, pp. 3374-3385, (2021) (PubMed).

Turnis, Kaminska, Smith, Kartchner, Vogel, Laxton, Ashmun, Ney, Opferman: "Requirement for antiapoptotic MCL-1 during early erythropoiesis." in: Blood, Vol. 137, Issue 14, pp. 1945-1958, (2021) (PubMed).

Djajawi, Liu, Gong, Huang, Luo, Xu, Okamoto, Call, Huang, van Delft: "MARCH5 requires MTCH2 to coordinate proteasomal turnover of the MCL1:NOXA complex." in: Cell death and differentiation, Vol. 27, Issue 8, pp. 2484-2499, (2021) (PubMed).

Moyzis, Lally, Liang, Leon, Najor, Orogo, Gustafsson: "Mcl-1-mediated mitochondrial fission protects against stress but impairs cardiac adaptation to exercise." in: Journal of molecular and cellular cardiology, Vol. 146, pp. 109-120, (2021) (PubMed).

Mizutani, Hikita, Saito, Myojin, Sato, Urabe, Kurahashi, Shiode, Sakane, Murai, Nozaki, Kodama, Sakamori, Yoshida, Tatsumi, Takehara: "Gab1 in livers with persistent hepatocyte apoptosis has an antiapoptotic effect and reduces chronic liver injury, fibrosis, and tumorigenesis." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 320, Issue 6, pp. G958-G968, (2021) (PubMed).

Sumiyoshi, Kotani, Ikuta, Suzue, Ozawa, Katakai, Yamada, Abe, Bando, Koyasu, Kanaho, Watanabe, Matsuda: "Arf1 and Arf6 Synergistically Maintain Survival of T Cells during Activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 206, Issue 2, pp. 366-375, (2021) (PubMed).

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Jayavelu, Schnöder, Perner, Herzog, Meiler, Krishnamoorthy, Huber, Mohr, Edelmann-Stephan, Austin, Brandt, Palandri, Schröder, Isermann, Edlich, Sinha, Ungelenk, Hübner, Zeiser, Rahmig, Waskow, Coldham, Ernst, Hochhaus, Jilg, Jost, Mullally, Bullinger, Me: "Splicing factor YBX1 mediates persistence of JAK2-mutated neoplasms." in: Nature, Vol. 588, Issue 7836, pp. 157-163, (2021) (PubMed).

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Target Details for MCL-1

Target: MCL-1 (MCL1) (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))

Alternative Name: Mcl-1 (MCL1 Products)

Background:

Synonyms: rabbit anti-Mcl-1 antibody, Mcl1, Mcl 1, Bcl 2 related protein EAT/mcl1 antibody, Bcl2 related antibody, EAT antibody, Induced myeloid leukemia cell differentiation protein Mcl-1 antibody

Background: Regulated apoptosis is essential for both the development and the subsequent maintenance of the immune system. Interleukins, including IL-2, IL-4, IL-7 and IL-15, heavily influence lymphocyte survival during the vulnerable stages of VDJ rearrangement and later in ensuring cellular homeostasis, but the genes specifically responsible for the development and maintenance of lymphocytes have not been identified. The Anti apoptotic protein Mcl-1 (myeloid cell leukemia sequence 1 (BCL2-related)) is an attractive candidate, as it is highly regulated, appears to enhance short-term survival and functions at an apical step in genotoxic deaths. However, Mcl-1 deficiency results in peri-implantation lethality. Mice, conditional for Mcl-1, display a profound reduction in B and T lymphocytes when Mcl-1 is removed. Deletion of Mcl-1 during early lymphocyte differentiation increases apoptosis and arrests the development at pro-B-cell and double negative T-cell stages. Induced deletion of Mcl-1 in peripheral B- and T-cell populations results in their rapid loss. Moreover, IL-7 both induces and requires Mcl-1 to mediate lymphocyte survival. Mcl-1 is essential both early in lymphoid development and later on in the maintenance of mature lymphocytes.

Gene Name: Mcl1

Gene ID: 17210

UniProt: P97287

Pathways: MAPK Signaling