CD25 antibody (PE)

Catalog No. ABIN6657778

Supplier Product No.: 200-508-m73

Product Details anti-CD25 Antibody (PE)

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD25 antibody is conjugated to PE

Application: Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Supplier Product No.: 200-508-m73

Supplier: Rockland

Purpose: CD25 Phycoerythrin Antibody

Cross-Reactivity (Details): Reactivity is observed against mouse CD25.

Purification: Phycoerythrin conjugated CD25 Monoclonal Antibody was Protein G Purified and is directed against mouse CD25.

Sterility: Sterile filtered

Immunogen: Anti-CD25 Antibody (Monoclonal) was produced by repeated immunizations with IL-2-dependent cytolytic mouse T-cell clone B6.1.

Clone: PC61

Isotype: IgG1 lambda

Labeling Ratio: 1-2

Application Details

Application Notes:

Flow_Cytometry_Dilution: 0.2-1 μg/1x10^6 cells

IF_Microscopy_Dilution: User Optimized

Comment:

Anti-CD25 is tested for FLOW and useful for IF. Researchers should determine optimal titers for applications that are not stated.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.1 % Gelatin

Preservative: 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Expiry Date: 6 months

Target Details for CD25

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Alternative Name: CD25 (IL2RA Products)

Background:

Synonyms: Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, TAC antigen, p55, CD25 Antibody

Background: The PC61.5 antibody is specific for mouse CD25, a 55 kDa surface protein also known as the Interleukin-2 Receptor alpha chain, or IL-2R alpha. CD25 may bind IL-2 by itself, although with low affinity and without induction of cell signaling. CD25 is also expressed within a high-affinity complex, along with the IL-2R beta chain (CD122) and the common gamma chain (CD132), to form a signaling receptor complex. Expression of CD25 varies during developmental stages of T and B cells, is induced on activated mature T and B cells, and is present on subsets of dendritic cells. CD25 signaling as part of the IL-2 receptor complex triggers T cell activation and proliferation, as well as modulating the differentiation and function of Th17 cells, T regulatory (Treg) cells, and dendritic cells. The PC61.5 antibody is used as a marker for T cells, B cells and dendritic cell subsets. Expression of CD25, CD4 and the transcription factor Foxp3 is regarded as a phenotypic signature for Treg cells. As such, this antibody is widely used for depletion of Treg cells in vivo, as well as to distinguish Treg cells from naive or conventional T cells which are CD25.

Gene Name: IL2RA

Gene ID: 16184

NCBI Accession: NP_032393

UniProt: P01590

Pathways: JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation