PIK3CD antibody (C-Term)

Catalog No. ABIN6657601

Supplier Product No.: 100-401-862

Product Details anti-PIK3CD Antibody

Target: PIK3CD (Phosphoinositide-3-Kinase, Catalytic, delta Polypeptide (PIK3CD))

Binding Specificity: C-Term

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIK3CD antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Supplier Product No.: 100-401-862

Supplier: Rockland

Purpose: PI3-kinase p110 delta Antibody

Cross-Reactivity (Details): Reactivity occurs against mouse PI3K p110d subunit.

Purification: This antibody was prepared from whole rabbit antiserum by delipidation and defibrination.

Sterility: Sterile filtered

Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal of mouse PI3K p110d. This sequence is identical in both mouse and human.

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:4,000 - 1:20,000

Western_Blot_Dilution: 1:5,000

Other: User Optimized

Comment:

Anti-PI3 kinase p110 delta subunit antibody has been tested for use in ELISA, western blotting and immunoprecipitation.  Reactivity in other immunoassays is unknown.  A mouse whole cell splenic lysate is suitable for use as a positive control.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-PIK3CD antibody (ABIN6657601)

Jou, Carpino, Takahashi, Piekorz, Chao, Carpino, Wang, Ihle: "Essential, nonredundant role for the phosphoinositide 3-kinase p110delta in signaling by the B-cell receptor complex." in: Molecular and cellular biology, Vol. 22, Issue 24, pp. 8580-91, (2003) (PubMed).

Target Details for PIK3CD

Target: PIK3CD (Phosphoinositide-3-Kinase, Catalytic, delta Polypeptide (PIK3CD))

Alternative Name: PI3-kinase p110 delta (PIK3CD Products)

Background:

Synonyms: rabbit anti-PI3-kinase p110 delta Antibody, p110D antibody, p110delta antibody, Phosphatidylinositol 3 kinase catalytic delta polypeptide antibody, Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit delta isoform antibody, PI3K antibody, PIK3CD antibody

Background: Phosphoinositide 3-kinases (PI3Ks) generate 3-phosphoinositide lipids in cell membranes.  A variety of intracellular target proteins interact with these lipids via specific lipid-binding modules and, as a consequence, undergo changes in their localization and/or activity. In this way, PI3Ks participate in the regulation of mitogenesis, differentiation, survival, intracellular vesicular transport, cytoskeletal reorganization, and motility.  Tyrosine kinases and Ras use PI3Ks as essential intracellular signal relay molecules. PI3Ks are heterodimeric enzymes consisting of a regulatory subunit in complex with a p110 catalytic subunit. Mammmals have genes encoding three distinct catalytic subunits (p110α, p110ß, and p110δ and three regulatory subunits (p85α, p85ß, and p55δ). All of the p110 isoforms are capable of interacting with each type of regulatory subunit. They are also similarly recruited to phosphotyrosine complexes and have, at least in vitro, the same lipid substrate specificity. However, it is becoming increasingly clear that PI3K isoforms differ in their interaction with Ras and regulation of lipid kinase activity, and in their protein kinase activities. Several groups have provided evidence that p110 isoforms have nonredundant functions in the regulation of cell proliferation, survival, actin cytoskeleton reorganization, and migration downstream of given receptors. This antibody is specific for the carboxy teminal end of p110d that is expressed predominantly in leukocytes.

Gene Name: PIK3CD

Gene ID: 18707

UniProt: O35904

Pathways: BCR Signaling, Warburg Effect