GLI1 antibody (AA 805-820)

Catalog No. ABIN6657594

Product Details anti-GLI1 Antibody

Target: GLI1 (Zinc Finger Protein GLI1 (GLI1))

Binding Specificity: AA 805-820

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLI1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Cross-Reactivity: Human, Mouse (Murine)

Purification: This whole rabbit antiserum was prepared by delipidation and defibrination followed by the addition of buffer salts and preservative.  Reactivity is observed against Mouse and Human Gli-1.  Cross-reactivity with other species is likely but has not been determined.  No reaction occurs with human or mouse Gli-2 or Gli-3.

Immunogen:

Immunogen: The whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to amino acids 805-820 of mouse Gli-1. The peptide was synthesized as a multiple antigen peptide (MAP).

Immunogen Type: Peptide

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:500 - 1:2,000

Application Note: This antibody has been tested for use in ELISA, immunofluorescence, and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 120 kDa in size corresponding to Gli-1 protein by western blotting in the appropriate cell lysate or extract.  For immunohistochemistry, perform heat mediated antigen retrieval via the microwave method before commencing with staining.

ELISA Dilution: 1:20,000 - 1:100,000

Western Blot Dilution: 1:2,000 - 1:10,000

IF Microscopy Dilution: 1:500 - 1:2,000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

0.01 % (w/v) Sodium Azide

Stabilizer: None

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

References for anti-GLI1 antibody (ABIN6657594)

Armas-López, Piña-Sánchez, Arrieta, de Alba, Ortiz-Quintero, Santillán-Doherty, Christiani, Zúñiga, Ávila-Moreno: "Epigenomic study identifies a novel mesenchyme homeobox2-GLI1 transcription axis involved in cancer drug resistance, overall survival and therapy prognosis in lung cancer patients." in: Oncotarget, Vol. 8, Issue 40, pp. 67056-67081, (2017) (PubMed).

Kotula, Sun, Li, Pratico, Fereshteh, Ahrens, Sullenger, Kovacs: "Targeted disruption of β-arrestin 2-mediated signaling pathways by aptamer chimeras leads to inhibition of leukemic cell growth." in: PLoS ONE, Vol. 9, Issue 4, pp. e93441, (2015) (PubMed).

Gurung, Feng, Hua: "Menin directly represses Gli1 expression independent of canonical Hedgehog signaling." in: Molecular cancer research : MCR, Vol. 11, Issue 10, pp. 1215-22, (2014) (PubMed).

Jalili, Mertz, Romanov, Wagner, Kalthoff, Stuetz, Pathria, Gschaider, Stingl, Wagner: "NVP-LDE225, a potent and selective SMOOTHENED antagonist reduces melanoma growth in vitro and in vivo." in: PLoS ONE, Vol. 8, Issue 7, pp. e69064, (2013) (PubMed).

Zhao, Ayrault, Zindy, Kim, Roussel: "Post-transcriptional down-regulation of Atoh1/Math1 by bone morphogenic proteins suppresses medulloblastoma development." in: Genes & development, Vol. 22, Issue 6, pp. 722-7, (2008) (PubMed).

Qualtrough, Buda, Gaffield, Williams, Paraskeva: "Hedgehog signalling in colorectal tumour cells: induction of apoptosis with cyclopamine treatment." in: International journal of cancer, Vol. 110, Issue 6, pp. 831-7, (2004) (PubMed).

Niemann, Unden, Lyle, Zouboulis, Toftgård, Watt: "Indian hedgehog and beta-catenin signaling: role in the sebaceous lineage of normal and neoplastic mammalian epidermis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100 Suppl 1, pp. 11873-80, (2003) (PubMed).

Wetmore, Eberhart, Curran: "The normal patched allele is expressed in medulloblastomas from mice with heterozygous germ-line mutation of patched." in: Cancer research, Vol. 60, Issue 8, pp. 2239-46, (2000) (PubMed).

Target Details for GLI1

Target: GLI1 (Zinc Finger Protein GLI1 (GLI1))

Alternative Name: Gli1 (GLI1 Products)

Synonyms: GLI antibody, Gli antibody, Gli-1 antibody, gli1.1 antibody, AV235269 antibody, Zfp-5 antibody, Zfp5 antibody, GLI family zinc finger 1 antibody, GLI family zinc finger 1 S homeolog antibody, GLI-Kruppel family member GLI1 antibody, GLI1 antibody, Gli1 antibody, gli1.S antibody, gli1 antibody

Background:

Synonyms: rabbit anti-Gli-1 Antibody, rabbit anti-Gli1 Antibody, Zinc finger protein GLI1 antibody, Glioma-associated oncogene antibody, Oncogene GLI antibody

Background: Anti Gli1 Antibody was produced against a peptide corresponding to the carboxy-terminal region of the mouse Gli-1 protein.  This region of Gli1 is not conserved among other gli family members, namely Gli-2 and Gli-3.  Gli was termed by Kinzler et al. (1987) as 'glioma-associated oncogene' amplified in malignant gliomas.  Analysis of the cloned gene demonstrates that the gene contains 5 repeats of zinc-finger sequences, which places Gli in the family of Kruppel (Kr) zinc finger proteins.  Northern analysis reveals that Gli is expressed in embryonal carcinoma cells but not in most adult tissue.  Gli has been localized to 12q13-q14.3 by Southern blot analysis.  In mice, the gene is located on chromosome 10.  In mice, three zinc finger transcription factors, Gli-1, Gli-2 and Gli-3, have been implicated in the transduction of Sonic hedgehog (Shh) signal.  In papillary epithelium, shh, gli1 and ptc all follow similar expression patterns.  Gli-1 expression is central and probably sufficient for tumor development in humans.

Gene Name: GLI1

Gene ID: 14632, 4885279

UniProt: P47806

Pathways: Hedgehog Signaling, Dopaminergic Neurogenesis