MYOD1 antibody (Internal Region)
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- Target See all MYOD1 Antibodies
- MYOD1 (Myogenic Differentiation 1 (MYOD1))
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Binding Specificity
- Internal Region
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Reactivity
- Mouse
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This MYOD1 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
- Cross-Reactivity
- Human
- Purification
- Anti-MyoD Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with Anti-MyoD from Human, Cat, Rat, and Mouse based on 100% homology with the immunizing sequence. Cross-reactivity with Anti-MyoD from other sources has not been determined.
- Immunogen
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Immunogen: MyoD Antibody was produced in mice prepared by repeated immunizations with recombinant mouse MyoD protein at an internal amino acid sequence.
Immunogen Type: Recombinant Protein
- Clone
- 5-8A
- Isotype
- IgG1
- Top Product
- Discover our top product MYOD1 Primary Antibody
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- Application Notes
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Immunohistochemistry Dilution: 5 μg/mL
Application Note: Anti-MyoD Antibody is suitable for use in IF/ICC, IHC (frozen), IP and WB. Expect a band approximately 45 kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.
Immunoprecipitation Dilution: 1 μg/mL
Western Blot Dilution: 1 μg/mL
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.20.02 % (w/v) Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- RT,4 °C,-20 °C
- Storage Comment
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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- Target
- MYOD1 (Myogenic Differentiation 1 (MYOD1))
- Alternative Name
- MyoD (MYOD1 Products)
- Synonyms
- MYF3 antibody, MYOD antibody, PUM antibody, bHLHc1 antibody, MyoD antibody, XMyoD antibody, XtmyoD antibody, mf25 antibody, myf3 antibody, pum antibody, MUF3 antibody, AI503393 antibody, Myod-1 antibody, etID309723.25 antibody, myod antibody, wu:fb57a01 antibody, zgc:136744 antibody, MYOD1 antibody, myod1 antibody, mf1 antibody, myod1-a antibody, myod1-b antibody, myogenic differentiation 1 antibody, MYOD protein antibody, myogenic factor MyoD1 antibody, myogenic differentiation 1 S homeolog antibody, myogenic differentiation 1 L homeolog antibody, MYOD1 antibody, myod1 antibody, Myod1 antibody, myod antibody, myod1.S antibody, myod1.L antibody
- Background
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Synonyms: Myod, Myoblast determination protein 1, Class C basic helix-loop-helix protein 1, bHLHc1, Myogenic factor 3, Myf-3
Background: Anti-MyoD Antibody detects MyoD. The Myogenic determination gene (MyoD) was first identified by the virtue of its ability to convert embryonic mouse fibroblast cells to muscle cells. It was subsequently shown that forced expression of MyoD (human homolog is myf 3) gene in a wide variety of normal and neoplastic cells could either convert the cells to muscle cells or activate a set of the otherwise transcriptionally inactive muscle-specific genes in these cells. The regulatory domain of the MyoD gene product lies within a 70 amino acid region and comprises a basic DNA binding motif and a helix-loop-helix (HLH) dimerization motif. Subsequent studies identified three other genes whose products shared sequence homology for the basic HLH domain of MyoD. These are: myf5, myogenin (human homolog is myf4) and myf6 (also known as MRF4 and herculin). Anti-MyoD Antibody is ideal for investigators involved in cell cycle protein, cytokines and growth factors.
Gene Name: Myod1
- Gene ID
- 17927
- NCBI Accession
- NP_034996
- UniProt
- P10085
- Pathways
- Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
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