Hemagglutinin antibody

Catalog No. ABIN6657573

Supplier Product No.: 200-301-975

Product Details anti-Hemagglutinin Antibody

Target: Hemagglutinin (HA)

Reactivity: Influenza A Virus H5N1

Virus Strain: A/Vietnam/1203/04

Host: Mouse

Clonality: Monoclonal

Conjugate: This Hemagglutinin antibody is un-conjugated

Application: ELISA, Immunoprecipitation (IP), Western Blotting (WB), Neutralization (Neut), Immunohistochemistry (IHC)

Supplier Product No.: 200-301-975

Supplier: Rockland

Purpose: H5N1 Antibody VN04-2

Cross-Reactivity (Details): VN04-2 monoclonal antibody did not cross-react with influenza viruses of other HA subtypes.

Purification: This product was purified from tissue culture supernatant fluid by Protein A chromatography and is specific for H5 hemagglutinin (HA) protein of influenza A virus [strain A/Vietnam/1203/04 (H5N1)].

Sterility: Sterile filtered

Immunogen: A/Vietnam/1203/04 Influenza Virus (VN04-2) antibody was produced by intraperitoneal immunization of BALB/c mice with concentrated purified virus preparation containing hemagglutinin (HA) protein of influenza A virus [strain A/Vietnam/1203/04 (H5N1)] using the modification of the method described by Kohler and Milstein. Each mouse received two immunizations of 15 mug HA with incomplete Freund's adjuvant, administered 3 week apart.

Clone: 15A3

Isotype: IgG2a kappa

Application Details

Application Notes:

Neutralization_Dilution: User Optimized

Immunoprecipitation_Dilution: User Optimized

ELISA_Dilution: 1:5,000

Immunohistochemistry_Dilution: User Optimized

Western_Blot_Dilution: User Optimized

Comment:

Suggested Applications: ELISA, FC, WB
Hemagglutinin of A/Vietnam/1203/04 Influenza Virus (VN04-2) monoclonal antibody can be used for hemagglutination inhibition (HI) assays to provide antigenic characterization of the influenza A viruses of the H5 HA subtype. This monoclonal antibody is suitable for virus neutralization assays (in cell culture and in embryonated chicken eggs), ELISA, immunoprecipitation, immunohistochemistry and western blotting.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-Hemagglutinin antibody (ABIN6657573)

Reed, Yen, DuBois, Bridges, Salomon, Webster, Russell: "Amino acid residues in the fusion peptide pocket regulate the pH of activation of the H5N1 influenza virus hemagglutinin protein." in: Journal of virology, Vol. 83, Issue 8, pp. 3568-80, (2009) (PubMed).

Govorkova, Webby, Humberd, Seiler, Webster: "Immunization with reverse-genetics-produced H5N1 influenza vaccine protects ferrets against homologous and heterologous challenge." in: The Journal of infectious diseases, Vol. 194, Issue 2, pp. 159-67, (2006) (PubMed).

Hanson, Boon, Lim, Webb, Ooi, Webby: "Passive immunoprophylaxis and therapy with humanized monoclonal antibody specific for influenza A H5 hemagglutinin in mice." in: Respiratory research, Vol. 7, pp. 126, (2006) (PubMed).

Hoffmann, Lipatov, Webby, Govorkova, Webster: "Role of specific hemagglutinin amino acids in the immunogenicity and protection of H5N1 influenza virus vaccines." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 36, pp. 12915-20, (2005) (PubMed).

Target Details for Hemagglutinin

Target: Hemagglutinin (HA)

Alternative Name: Hemagglutinin (HA Products)

Target Type: Influenza Protein

Background:

Synonyms: mouse anti-H5N1 Antibody, mouse anti-VN04-2 Antibody, H5HA antibody, Hemagglutinin 5 antibody, H5N1 antibody

Background: Hemagglutinin of A/Vietnam/1203/04 Influenza Virus (VN04-2) Antibody raised against the hemagglutinin (HA) surface glycoprotein of the A/Vietnam/1203/04 (H5N1) influenza virus. Generally referred to as ''bird flu'', the H5N1 influenza A virus has been documented in poultry and humans across ten Eurasian countries, from Japan in the north to Indonesia in the south. Without immunity, humans would have no protection against H5N1 influenza viruses, which could potentially cause a catastrophic pandemic influenza.  This antibody, directed against the HA surface glycoprotein of the A/Vietnam/1203/04 (H5N1) influenza virus, is intended to further our understanding of the mechanisms underlying antigenic variation and evolution of novel variants. The major functions of HA include receptor-binding and fusion activities, but there may also be a structural role for HA in viral particle formation. Following attachment of HA to surface receptors on susceptible cells, the influenza virus enters the cell via endocytosis and membrane fusion.

Gene Name: HA

Gene ID: 159144921

UniProt: A8UDQ2