ATM antibody (pSer1981)

Catalog No. ABIN6657494

Supplier Product No.: 200-301-400s

Product Details anti-ATM Antibody

Target: ATM (Ataxia Telangiectasia Mutated (ATM))

Binding Specificity: AA 1974-1988, pSer1981

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ATM antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Supplier Product No.: 200-301-400s

Supplier: Rockland

Purpose: ATM phospho S1981 Antibody

Cross-Reactivity (Details): This monoclonal anti-ATM antibody recognizes the phosphorylated epitope in native and over-expressed proteins found in various tissues and extracts.

Purification: Anti-ATM phospho S1981 Monoclonal Antibody is directed against human ATM and is useful in determining its presence in various assays.

Sterility: Sterile filtered

Immunogen: Anti-ATM phospho S1981 Antibody was produced from a synthetic peptide S-L-A-F-E-E-G-Sp-Q-S-T-T-I-S-S corresponding to aa 1974-1988 of human ATM.

Clone: 10H11-E12

Isotype: IgG1 kappa

Application Details

Application Notes:

Immunoprecipitation_Dilution: User Optimized

ELISA_Dilution: 1:20,000 - 1:100,000

Immunohistochemistry_Dilution: Not Recommended

Flow_Cytometry_Dilution: 5 μg/mL

IF_Microscopy_Dilution: 1:100 - 1:500

Western_Blot_Dilution: 1:200 - 1:2,000

Other: User Optimized

Comment:

Suggested Applications:

Suggested_Applications: Biochemical Assay, ChIP, FISH, IHC, IP, Multiplex

Other_Performance_Data: iFISH at 7.5μg/ml

Protein A Purified Mab anti-ATM has been tested by ELISA, FC, IF, and Western blotting against both the native and recombinant forms of the protein. The antibody immunoprecipitates ATM from irradiated human and transfected mouse cells. By immunofluorescence, foci are detected in irradiated human and mouse fibroblasts. This antibody is not recommended for immunohistochemistry. Instead, for IHC, use the clone 7C10D8 (item# 200-301-500).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

References for anti-ATM antibody (ABIN6657494)

Kreis, Gallrein, Rojas-Puente, Mack, Kroon, Dinkel, Willmes, Murk, Tom-Dieck, Schuman, Kirstein, Eickholt: "ATM phosphorylation of the actin-binding protein drebrin controls oxidation stress-resistance in mammalian neurons and C. elegans." in: Nature communications, Vol. 10, Issue 1, pp. 486, (2019) (PubMed).

Carvalho, Vítor, Sridhara, Martins, Raposo, Desterro, Ferreira, de Almeida: "SETD2 is required for DNA double-strand break repair and activation of the p53-mediated checkpoint." in: eLife, Vol. 3, pp. e02482, (2015) (PubMed).

Bartkova, Horejsí, Koed, Krämer, Tort, Zieger, Guldberg, Sehested, Nesland, Lukas, Ørntoft, Lukas, Bartek: "DNA damage response as a candidate anti-cancer barrier in early human tumorigenesis." in: Nature, Vol. 434, Issue 7035, pp. 864-70, (2005) (PubMed).

Target Details for ATM

Target: ATM (Ataxia Telangiectasia Mutated (ATM))

Alternative Name: ATM (ATM Products)

Background:

Synonyms: mouse anti-ATM antibody, mouse anti-ATMpS1981 antibody, mouse anti- ATM pS1981 antibody, DKFZp781A0353 antibody, Human phosphatidylinositol 3 kinase homolog antibody, MGC74674 antibody, Serine protein kinase ATM antibody, T cell prolymphocytic leukemia antibody

Background: Anti ATM pS1981 Antibody is a phospho site specific antibody and recognizes the product of the ATM gene that is mutated in the hereditary disease ataxia-telangiectasia. ATM codes for a protein kinase that acts as a master regulator of cellular responses to DNA double-strand breaks. ATM is normally inactive and the question of how it is activated in the event of DNA damage (due to ionizing radiation for instance) is central to understanding its function. ATM protein is now shown to be present in undamaged cells as an inactive dimer. Low doses of ionizing radiation, which induce only a few DNA breaks, activate at least half of the total ATM protein present, possibly in response to changes in chromatin structure. The ATM gene encodes a 370- kDa protein that belongs to the phosphoinositide 3-kinase (PI(3)K) superfamily, but which phosphorylates proteins rather than lipids. The 350-amino-acid kinase domain at the carboxy terminus of this large protein is the only segment of ATM with an assigned function. Exposure of cells to IR triggers ATM kinase activity, and this function is required for arrests in G1, S and G2 phases of the cell cycle. Several substrates of the ATM kinase participate in these IR-induced cell-cycle arrests. These include p53, Mdm2 and Chk2 in the G1 checkpoint, Nbs1, Brca1, FancD2 and SMC1 in the transient IR-induced S-phase arrest, and Brca1 and hRad17 in the G2/M checkpoint. Ideal for Cancer, Cell Signaling, Chromatin, Neuroscience and Signal Transduction research.

Gene Name: ATM

Gene ID: 472

NCBI Accession: NP_000042

UniProt: Q13315

Pathways: p53 Signaling, Apoptosis, DNA Damage Repair, Inositol Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus