8-OHDG antibody

Catalog No. ABIN6657492

Product Details anti-8-OHDG Antibody

Target: 8-OHDG (8-Hydroxyguanosine (8-OHDG))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This 8-OHDG antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Purification: This Protein G purified Anti-8-Hydroxy Guanine monoclonal antibody recognizes markers of oxidative damage to DNA (8-hydroxy-2'-deoxyguanosine, 8-hydroxyguanine and 8-hydroxyguanosine).

Immunogen:

Immunogen: This Protein G purified monoclonal antibody was prepared using conventional hybridoma technology after repeated immunizations with 8-hydroxy-guanosine-BSA and casein conjugates.

Immunogen Type: Native Protein

Clone: 15A3

Isotype: IgG2b

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:1000

Application Note: This Protein G purified antibody has been tested for use in immunohistochemistry, ICC/IF, Dot Blot, IP, Flow Cytometry, and ELISA.  Specific conditions for reactivity should be optimized by the end user.

Immunoprecipitation Dilution: User Optimized

ELISA Dilution: User Optimized

Flow Cytometry Dilution: User Optimized

IF Microscopy Dilution: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

0.1 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store Anti-8-Hydroxy Guanine antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

References for anti-8-OHDG antibody (ABIN6657492)

Neumann, Lenz, Streit, Bechmann: "Is microglial dystrophy a form of cellular senescence? An analysis of senescence markers in the aged human brain." in: Glia, Vol. 71, Issue 2, pp. 377-390, (2022) (PubMed).

Jabir, Hopkins, Ritchie, Ullah, Bayes, Li, Tourlomousis, Lupton, Puleston, Simon, Bryant, Evans: "Mitochondrial damage contributes to Pseudomonas aeruginosa activation of the inflammasome and is downregulated by autophagy." in: Autophagy, Vol. 11, Issue 1, pp. 166-82, (2015) (PubMed).

Target Details for 8-OHDG

Target: 8-OHDG (8-Hydroxyguanosine (8-OHDG))

Alternative Name: 8-Hydroxy Guanine (8-OHDG Products)

Target Type: Chemical

Background:

Synonyms: 8 hydroxy 2' deoxyguanosine antibody, 8 hydroxyguanine antibody, 8 hydroxyguanosine antibody, 8 OHG antibody, 8-OHG antibody, 8OG antibody, 8OHdG antibody, 8OHG antibody, 8-Hydroxy Guanine Antibody, 8-OH-dG Antibody, DNA/RNA Damage Antibody

Background: DNA or RNA damage is due to environmental factors and normal metabolic processes inside the cell, that then hinder the ability of the cell to carry out its functions. There are four main types of DNA damage due to endogenous cellular processes: oxidation, alkylation, hydrolysis and mismatch of the bases. During the oxidation of bases, highly reactive chemical entities collectively known as RONS may develop. RONS stands for reactive oxygen and nitrogen species and includes nitric oxide, superoxide, hydroxyl radical, hydrogen peroxide and peroxynitrite. Numerous studies have shown that RONS cause a variety of other issues in addition to DNA damage. 8-hydroxyguanine, 8-hydroxy-2'-deoxyguanosine and 8-hydroxyguanosine are all RNA and DNA markers of oxidative damage. 8-hydroxy-2'-guanosine is produced by reactive oxygen and nitrogen species including hydroxyl radical and peroxynitrite. Specifically its high biological relevance is due to its ability to induce G to T transversions, which is one of the most frequent somatic mutations (2). 8-hydroxy-guanine has been the most frequently studied type of DNA base damage, with studies in diabetes, and cancer. Base modifications of this type arise from radical-induced hydroxylation and cleavage reactions of the purine ring. Finally, 8-hydroxy-guanosine, like 8-hydroxy-2'-guanosine, induces a mutagenic transversion of G to T in DNA. Its role has been tested specifically in the development of diabetes, hypertension and strokes.