Bassoon antibody (N-Term)

Catalog No. ABIN6657297

Product Details anti-Bassoon Antibody

Target: Bassoon (BSN)

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Bassoon antibody is un-conjugated

Application: Western Blotting (WB), Fluorescence Microscopy (FM)

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Purification: Anti-Bassoon Antibody was purified by affinity chromatography. A BLAST analysis was used to suggest cross-reactivity with Bassoon from Human, Mouse, and Rat based on 100% homology with the immunizing sequence. Other species not yet tested. Cross-reactivity with Bassoon from other sources has not been determined. Neuroscience research.

Immunogen:

Immunogen: Bassoon Antibody was produced from whole rabbit serum prepared by repeated immunizations with a N-terminal his-tagged fusion protein.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Bassoon Antibody is suitable for use in WB and IHC. Expect a band approximately ~420 kDa on specific lysates corresponding to the molecular mass of Bassoon. Multiple isoforms can be detected. Specific conditions for reactivity should be optimized by the end user. Product provided in PBS pH 7.4.

Western Blot Dilution: 1:1000

IF Microscopy Dilution: 1:400

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer:

Stabilizer: 50 % (v/v) Glycerol

0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Target Details for Bassoon

Target: Bassoon (BSN)

Alternative Name: Bassoon (BSN Products)

Synonyms: Znf231 antibody, ZNF231 antibody, bassoon antibody, bassoon (presynaptic cytomatrix protein) antibody, bassoon presynaptic cytomatrix protein antibody, Bsn antibody, BSN antibody

Background:

Synonyms: BSN, ZNF231, Neuronal double zine finger protein, protein bassoon

Background: Bassoon is a 420 kDa protein that is a localized at the presynaptic nerve terminals and is believed to play a role in the structural and functional organization of the synaptic vesicle cycle. Bassoon is predicted to contain two double-zinc fingers, three coiled-coil regions, and two polyglutamine domains. The polyglutamine domains in the C-terminus are of interest, since it is known that for some human proteins, such as Huntingtin, abnormal amplification of this region can cause late-onset neurodegeneration. Bassoon is concentrated at sites opposite to postsynaptic densities in synaptic terminals and in cultured neurons, it is found to colocalize with GABA (A) and glutamate (GluR1) receptors.

Gene Name: BSN

Gene ID: 8927

NCBI Accession: NP_003449

UniProt: Q9UPA5