AQP2 antibody (C-Term)

Catalog No. ABIN6656925

Supplier Product No.: 600-401-e90

Product Details anti-AQP2 Antibody

Target: AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))

Binding Specificity: C-Term

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AQP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Supplier Product No.: 600-401-e90

Supplier: Rockland

Purpose: Aquaporin 2 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with Aquaporin 2 from Human, Mouse, and Rat based on 100 % homology with the immunizing sequence.

Purification: Anti-Aquaporin 2 Antibody was purified by affinity chromatography.

Sterility: Sterile filtered

Immunogen: Aquaporin 2 Antibody was produced from whole rabbit serum prepared by repeated immunizations with a synthetic peptide corresponding to the C-terminal region of rat aquaporin 2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry_Dilution: 1:200

IF_Microscopy_Dilution: 1:400

Western_Blot_Dilution: 1:1000 - 1:4000

Comment:

Anti-Aquaporin 2 Antibody is tested for use in WB, IHC, and IF microscopy. Expect a band approximately ~28.5kDa on specific lysates. May detect larger glycosylated bands ~35-50kDa. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.1 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for AQP2

Target: AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))

Alternative Name: Aquaporin 2 (AQP2 Products)

Background:

Synonyms: AQP2, AQP-CD, AQPCD, WCH CD, Aquaporin-2, AQP-2, ADH water channel, Aquaporin-CD, QP-CD, Collecting duct water channel protein, WCH-CD, Water channel protein for renal collecting duct

Background: Aquaporins selectively conduct water molecules in and out of the cell, while preventing the passage of ions and other solutes. Known as water channels, they are integral membrane pore proteins. Aquaporin 2 is the vasopressin-regulated water channel of the apical membrane of collecting duct cells. It is located in kidney epithelial cells and usually lies dormant in intracellular vesicle membranes. When it is needed vasopressin binds to the cell surface vasopressin receptor, activating a signaling pathway that cause AQP2 containing vesicles to fuse with the plasma membrane so the AQP2 can be used by the cell. Defects in AQP2 area cause of an autosomal dominant form of nephrogenic diabetes insipidus (NDI).

Gene Name: Aqp2

Gene ID: 25386

NCBI Accession: NP_037041

UniProt: P34080

Pathways: Response to Water Deprivation