Aquaporin 1 antibody (N-Term)

Catalog No. ABIN6656923

Supplier Product No.: 600-401-e89

Product Details anti-Aquaporin 1 Antibody

Target: Aquaporin 1 (AQP1) (Aquaporin 1 (Colton Blood Group) (AQP1))

Binding Specificity: N-Term

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aquaporin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Supplier Product No.: 600-401-e89

Supplier: Rockland

Purpose: Aquaporin 1 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with Aquaporin 1 from Human, Mouse, and Rat based on 100 % homology with the immunizing sequence.

Purification: Anti-Aquaporin 1 Antibody was purified by affinity chromatography.

Sterility: Sterile filtered

Immunogen: Aquaporin 1 Antibody was produced from whole rabbit serum prepared by repeated immunizations with a synthetic peptide corresponding to the N-terminal region of rat aquaporin 1.

Isotype: IgG

Application Details

Application Notes:

IF_Microscopy_Dilution: 1:200-400

Western_Blot_Dilution: 1:1000-4000

Comment:

Anti-Aquaporin 1 Antibody is tested for use in WB, IHC, and IF microscopy. Expect a band approximately ~28.5kDa on specific lysates. May detect larger glycosylated bands ~35-50kDa. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.1 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Aquaporin 1

Target: Aquaporin 1 (AQP1) (Aquaporin 1 (Colton Blood Group) (AQP1))

Alternative Name: Aquaporin 1 (AQP1 Products)

Background:

Synonyms: AQP1, Water channel protein CHIP29, Aquaporin-CHIP, Water channel protein for red blood cells and kidney proximal tubule, Aquaporin-1, Chip28

Background: Aquaporins selectively conduct water molecules in and out of the cell, while preventing the passage of ions and other solutes. Known as water channels, they are integral membrane pore proteins. Aquarporin 1 is a widely expressed water channel, found in the basolateral and apical plasma membranes of the proximal tubes, the descending loop of Henel and in the descending portion of the vasa recta. Additionally it is found in red blood cells, vascular endothelium, gastrointestinal tract, sweat glands and lungs. It is not regulated by vasopressin.

Gene Name: Aqp1

Gene ID: 25240

NCBI Accession: NP_036910

UniProt: P29975

Pathways: Hormone Transport