TLR4 antibody

Catalog No. ABIN6656855

Supplier Product No.: 200-301-i25

Product Details anti-TLR4 Antibody

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TLR4 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Fluorescence Microscopy (FM), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB)

Supplier Product No.: 200-301-i25

Supplier: Rockland

Purpose: TLR4 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with Anti-TLR4 from human and mouse based on 100 % homology with the immunizing sequence.

Purification: Anti-TLR4 Antibody was purified by Protein G chromatography.

Immunogen: TLR4 Antibody was produced in mice prepared by repeated immunizations with amino acids corresponding to an internal sequence in human TLR4.

Clone: 76B357-1

Isotype: IgG2b kappa

Application Details

Application Notes:

Immunohistochemistry_Dilution: 5 μg/mL

Flow_Cytometry_Dilution: 0.5-2.0 μg/10^6 cells

IF_Microscopy_Dilution: 1:10-1:2000

Western_Blot_Dilution: 1-3 μg/mL

Comment:

Anti-TLR4 Antibody has been tested in WB, ChIP, DB, ELISA, Flow, Flow-CS, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, CyTOF-ready, Dual ISH-IHC, ELISA(Capture), and Knockdown/Knockout. Expect a band approximately 95kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.05 % BSA

Preservative: 0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for TLR4

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Alternative Name: TLR4 (TLR4 Products)

Background:

Synonyms: hToll, Toll-like receptor 4, CD284

Background: Anti-TLR4 CD284 antibody detects human TLR4 CD284. The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described. Among this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediators of LPS signaling. The TLR4 cDNA codes for a protein consisting of 799 amino acids with approximate molecular weight of 88 kDa. Anti-TLR4 CD284 antibody is ideal for investigators involved in cytokines and growth factor research.

Gene Name: TLR4

Gene ID: 7099

NCBI Accession: NP_003257

UniProt: O00206

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome, S100 Proteins