Reticulon 4 antibody

Catalog No. ABIN6656584

Supplier Product No.: 100-401-h97

Product Details anti-Reticulon 4 Antibody

Target: Reticulon 4 (RTN4)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Reticulon 4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Supplier Product No.: 100-401-h97

Supplier: Rockland

Purpose: Nogo-A B Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with Anti-Nogo-A B from human, mouse, and rat based on 100 % homology with the immunizing sequence.

Purification: Anti-Nogo A/B Antibody is sera.

Immunogen: Nogo-A/B Antibody was produced from whole rabbit serum prepared by repeated immunizations with a synthetic sequence corresponding to internal Nogo-A human protein.

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: User Optimized

Immunohistochemistry_Dilution: 1:100

IF_Microscopy_Dilution: User Optimized

Western_Blot_Dilution: 1:500-1:2000

Comment:

Anti-Nogo A/B Antibody has been tested by WB, ELISA, ICC/IF, IHC, IHC-P. Expect a band approximately 48-50kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.05 % BSA

Preservative: 0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store Nogo A B antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Reticulon 4

Target: Reticulon 4 (RTN4)

Alternative Name: Nogo-A B (RTN4 Products)

Background:

Synonyms: KIAA0886, NOGO, Reticulon-4, Foocen, Neurite outgrowth inhibitor, Nogo protein, Neuroendocrine-specific protein, NSP, Neuroendocrine-specific protein C homolog, RTN-x, Reticulon-5

Background: Nogo B 1/2 is a novel myelin-associated, intermediate-length splice variant of Nogo belonging to the reticulon family. It is a multi domain neurite growth inhibitory protein containing several discrete regions with growth inhibitory functions and is a component of the CNS myelin that prevents axonal regeneration in the adult vertebrate CNS. Nogo B1/2 along with NGBR mediated chemotaxis in HUVECs and induced tube formation in 3-dimensional cultures. It is mostly found in intact blood vessels, smooth muscle cells, and endothelial cell, with its N-terminus oriented extracellularly and is a regulator of vascular homeostasis and remodeling. It plays an important role in sporadic and familial amyotrophic lateral sclerosis. Anti-Nogo A/B is ideal for researchers interested in Neurobiology research.

Gene Name: RTN4

Gene ID: 57142

NCBI Accession: NP_008939

UniProt: Q9NQC3

Pathways: Neurotrophin Signaling Pathway, Regulation of Cell Size, SARS-CoV-2 Protein Interactome