Cyclin L1/L2 antibody

Catalog No. ABIN6656457

Product Details anti-Cyclin L1/L2 Antibody

Target: Cyclin L1/L2 (CCNL1/2)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Application: Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Purification: The product was purified from monospecific antiserum by Protein A chromatography. This antibody will react with all forms of Cyclin L b (cyclin L1b and cyclin L2b).   Cross reactivity with Cyclin L beta proteins from other sources is unknown.

Immunogen:

Immunogen: This Protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic construct consisting of full length Human Cyclin L1b protein.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 2 mg/mL - 20 μg/mL

Application Note: This Protein A purified antibody has been tested for use in ELISA, western blot, immunohistochemistry, and immunoprecipitation.   Specific conditions for reactivity should be optimized by the end user.  Expect a band ~ 25 kDa to 35 kDa in size corresponding to Cyclin L1b and Cyclin L2b by western blotting in the appropriate cell lysate or extract.

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:25,000

Western Blot Dilution: 1:1,000 - 1:5,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

0.01 % (w/v) Sodium Azide

Stabilizer: None

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

References for anti-Cyclin L1/L2 antibody (ABIN6656457)

Loyer, Trembley, Katona, Kidd, Lahti: "Role of CDK/cyclin complexes in transcription and RNA splicing." in: Cellular signalling, Vol. 17, Issue 9, pp. 1033-51, (2005) (PubMed).

Target Details for Cyclin L1/L2

Target: Cyclin L1/L2 (CCNL1/2)

Alternative Name: Cyclin L1/L2

Background:

Synonyms: rabbit anti-Cyclin L1/L2 Antibody, rabbit anti-Cyclin L1 Antibody, rabbit anti-Cyclin L2 Antibody, Cyclin L beta, CCNL1, CCNL2, Paneth cell-enhanced expression protein

Background: Cyclin L (also referred to as CCNL) is encoded by two highly related genes Cyclin L1 and Cyclin L2 (CCNL1 and CCNL2, respectively). Cyclin L has been shown to associate with the PITSLRE kinase and is involved in pre-mRNA processing. Both Cyclin L gene products can be alternatively spliced to produce two isoforms (known as 1 and 2 or alpha and beta). Cyclin L2 is ubiquitous, expressed at much higher levels than Cyclin L1, and thus is likely the major partner for the CDK11p110 protein kinase.

Gene Name: CCNL1, CCNL2

Gene ID: 57018

NCBI Accession: NP_064703

UniProt: Q9UK58