HAPLN2 antibody (Internal Region)

Catalog No. ABIN6656220

Product Details anti-HAPLN2 Antibody

Target: HAPLN2 (Hyaluronan and Proteoglycan Link Protein 2 (HAPLN2))

Binding Specificity: Internal Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HAPLN2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Cross-Reactivity (Details): Cross reactivity with BRAL1 from other sources has not been determined.

Purification: Anti-BRAL1 Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Immunogen:

Immunogen: Anti-BRAL1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 12 amino acid synthetic peptide from near the internal region of human BRAL1.

Immunogen Type: Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 2.5 μg/mL

Application Note: Anti-BRAL1 Antibody has been tested for use in ELISA, Western Blotting, Immunohistochemistry and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 35 kDa in Western Blots of specific cell lysates and tissues.

ELISA Dilution: 1:10,000-1:20,000

Western Blot Dilution: 1 - 2 μg/mL

IF Microscopy Dilution: 20 μg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

0.02 % (w/v) Sodium Azide

Stabilizer: None

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Target Details for HAPLN2

Target: HAPLN2 (Hyaluronan and Proteoglycan Link Protein 2 (HAPLN2))

Alternative Name: BRAL1 (HAPLN2 Products)

Synonyms: HAPLN2 antibody, fj51g03 antibody, wu:fj51g03 antibody, si:dkey-235h8.3 antibody, BRAL1 antibody, 4930401E20Rik antibody, Bral1 antibody, hyaluronan and proteoglycan link protein 2 antibody, HAPLN2 antibody, hapln2 antibody, Hapln2 antibody

Background:

Synonyms: BRAL1 Antibody, BRAL1, Hyaluronan and proteoglycan link protein 2, Brain link protein 1

Background: BRAL1 is a member a superfamily consisting of several highly homologous hyaluronan and proteoglycan binding link proteins. BRAL1 is predominantly expressed in brain tissue and spinal cord. Like other members in the link-module superfamily, BRAL1 contains an immunoglobulin-like fold and two proteoglycan tandem repeats (PTRs). Its mRNA expression pattern is similar to other lectican proteoglycans, suggesting that BRAL1 may act to stabilize the binding between the extracellular matrix molecule hyaluronan and brevican. Immunostaining of mouse brain showed BRAL1 expression at P20 in the white matter of the developing cerebellum and in myelinated fiber tracts in the adult brain, suggesting that expression starts when axonal myelination occurs.

Gene Name: HAPLN2

Gene ID: 60484

NCBI Accession: NP_068589

UniProt: Q9GZV7